Gadoxetate acid-enhanced MRI of hepatocellular carcinoma in a c-myc/TGFα transgenic mouse model including signal intensity and fat content: initial experience.
ABSTRACT Genetically engineered mouse models, such as double transgenic c-myc/TGFα mice, with specific pathway abnormalities might be more successful at predicting the clinical response of hepatocellular carcinoma (HCC) treatment. But a major drawback of the tumour models is the difficulty of visualizing endogenously formed tumours. The optimal imaging procedure should be brief and minimally invasive. Magnetic resonance imaging (MRI) satisfies these criteria and gadoxetate acid-enhanced MRI improves the detection of HCC. Fat content is stated to be an additional tool to help assess tumour responses, for example, in cases of radiofrequency ablation. Therefore the aim of this study was to investigate if gadoxetate acid-enhanced MRI could be used to detect HCC in c-myc/TGFα transgenic mice by determining the relation between the signal intensity of HCC and normal liver parenchyma and the corresponding fat content as a diagnostic marker of HCC. In our study, 20 HCC in c-myc/TGFα transgenic male mice aged 20-34 weeks were analyzed. On gadoxetate acid-enhanced MRI, the signal intensity was 752.4 for liver parenchyma and 924.5 for HCC. The contrast to noise ratio was 20.4, the percentage enhancement was 267.1% for normal liver parenchyma and 353.9% for HCC. The fat content was 11.2% for liver parenchyma and 16.2% for HCC. There was a correlation between fat content and signal intensity with r = 0.7791. All parameters were statistically significant with P < 0.05. Our data indicate that gadoxetate acid contrast enhancement allows sensitive detection of HCC in c-myc/TGFα transgenic mice and determination of the fat content seems to be an additional useful parameter for HCC.
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ABSTRACT: To clarify the factors that predict enhancement of the liver parenchyma in hepatocyte-phase of gadolinium ethoxybenzyl diethylenetriaminepentaacetic acid (Gd-EOB-DTPA)-enhanced MR imaging. Gd-EOB-DTPA-enhanced hepatocyte-phase MR images of 198 patients with chronic liver diseases (Child-Pugh class A in 112 patients, class B in 74 patients, and class C in 12 patients) were retrospectively analyzed. The hepatocyte-phase images were obtained using fat-suppressed T1-weighted gradient-echo images with a 3D acquisition sequence 10 min and 20 min after IV administration of Gd-EOB-DTPA (0.025 mmol/kg body weight). The quantitative liver-spleen contrast ratio (Q-LSC) was calculated using the signal intensities of the liver and spleen. Serum albumin levels, total bilirubin levels, prothrombin activity, and the results of indocyanine green clearance tests (ICGs) were recorded and correlated with the Q-LSC. Logistic regression analysis was performed to analyze which factors predict sufficient liver enhancement using a Q-LSC of 1.5 as a cutoff value. Only ICGs and Child-Pugh classifications showed a statistically significant correlation with the Q-LSC. Logistic regression analysis showed that ICGs were the only factors that accurately predicted liver enhancement on hepatocyte-phase images. ICGs were found to be predictors of sufficient liver enhancement on hepatocyte-phase images.Journal of Magnetic Resonance Imaging 11/2009; 30(5):1042-6. · 2.57 Impact Factor
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ABSTRACT: Characteristics of hepatocellular carcinoma (HCC) complicating nonalcoholic steatohepatitis (NASH) are still controversial. Most NASH related HCCs are believed to develop from cirrhotic liver, but case reports about HCC arising from non-cirrhotic NASH have been accumulating recently. This study is designed to elucidate characteristics of NASH related HCC diagnosed with high accuracy by using surgically resected specimens that contain larger areas to validate than biopsy specimens. For this study, 1168 patients who underwent hepatic resection at Osaka Medical Center for Cancer and Cardiovascular Diseases were enrolled. Patients who had clinically obvious causes of chronic liver dysfunction, such as viral and alcoholic hepatitis, were excluded. Histological diagnosis of NASH was confirmed according to Brunt's criterion. Eight (1%) patients were diagnosed with NASH. Six (75%) of them showed non-cirrhosis in non-cancerous areas. Stages of fibrosis in the non-cirrhotic patients were mild fibrosis (F2) in five and moderate fibrosis (F3) in one. All patients complicated with metabolic diseases. Although all these patients without cirrhosis were evaluated pathologically at tumor-node-metastasis stages I or II, three (50%) had multiple recurrences of tumor within a short time after surgery. This study indicates HCC might arise frequently from non-cirrhotic NASH. While further studies are needed to confirm this observation, both cirrhotic and non-cirrhotic NASH warrant regular screening for HCC.Journal of Gastroenterology 09/2009; 44(12):1190-4. · 4.02 Impact Factor
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ABSTRACT: The objective of our study was to prospectively evaluate quantitatively and qualitatively the enhancement patterns of cirrhotic liver tissue and hepatocellular carcinoma (HCC) after administration of the hepatocyte-specific contrast agent gadolinium ethoxybenzyl diethylenetriamine pentaacetic acid (Gd-EOB-DTPA) on dynamic MRI and to determine the time point of maximum liver-to-lesion contrast. Twenty-five patients with HCC in liver cirrhosis underwent 1.5-T MRI. T2-weighted turbo spin-echo and T1-weighted 3D gradient-echo sequences before and between 15 seconds and 20 minutes after the injection of 10 mL of Gd-EOB-DTPA were performed. Signal-to-noise ratios (SNRs) of liver parenchyma and liver-to-lesion contrast-to-noise ratios (CNRs) were calculated and plotted over time. Enhancement patterns of HCC were characterized qualitatively by two radiologists. The SNR of liver parenchyma increased significantly at 15 seconds and 60 seconds after contrast injection and remained stable thereafter. HCC showed positive CNR during the arterial phase and increasingly negative CNR during the further time course (p < 0.05). The maximum absolute CNR was found at 20 minutes after contrast injection. There was no correlation between the degree of enhancement at any time point and tumor grade. On qualitative evaluation, 16 HCCs showed arterial enhancement with early washout, and five showed arterial enhancement with late washout. In the remaining four HCCs, enhancement persisted until 20 minutes. Lesion conspicuity at 20 minutes after contrast injection was at least equal to or higher than it was on the remaining sequences in 19 of the 25 patients. After Gd-EOB-DTPA injection, most HCCs showed typical arterial enhancement with early washout. Liver-to-lesion contrast was best at 20 minutes.American Journal of Roentgenology 10/2009; 193(4):1053-60. · 2.74 Impact Factor
Cancer Imaging (2012) 12, 72?78
Gadoxetate acid-enhanced MRI of hepatocellular
carcinoma in a c-myc/TGF? transgenic mouse model
including signal intensity and fat
content: initial experience
Huedayi Korkusuza, Lea Knaub, Wolfgang Kromenb, Frank Huebnerb, Renate Hammerstinglb,
Sebastian Lindemayrb, Verena Bihrerc, Albrecht Piiperc, Thomas J Voglb
aDepartment of Nuclear Medicine, Johann Wolfgang Goethe University Hospital, Theodor-Stern-Kai 7,
D-60590 Frankfurt, Germany;bDepartment of Diagnostic and Interventional Radiology,
Johann Wolfgang Goethe University Hospital, Theodor-Stern-Kai 7, D-60590 Frankfurt, Germany;
cDepartment of Medicine I, Johann Wolfgang Goethe University Frankfurt, Theodor-Stern-Kai 7,
60590 Frankfurt, Germany
Corresponding address: Huedayi Korkusuz, Department of Nuclear Medicine, Johann Wolfgang Goethe University,
Theodor-Stern-Kai 7, 60590 Frankfurt, Germany.
Date accepted for publication 9 December 2011
Genetically engineered mouse models, such as double transgenic c-myc/TGF? mice, with specific pathway abnorm-
alities might be more successful at predicting the clinical response of hepatocellular carcinoma (HCC) treatment. But
a major drawback of the tumour models is the difficulty of visualizing endogenously formed tumours. The optimal
imaging procedure should be brief and minimally invasive. Magnetic resonance imaging (MRI) satisfies these criteria
and gadoxetate acid-enhanced MRI improves the detection of HCC. Fat content is stated to be an additional tool to
help assess tumour responses, for example, in cases of radiofrequency ablation. Therefore the aim of this study was to
investigate if gadoxetate acid-enhanced MRI could be used to detect HCC in c-myc/TGF? transgenic mice by
determining the relation between the signal intensity of HCC and normal liver parenchyma and the corresponding
fat content as a diagnostic marker of HCC. In our study, 20 HCC in c-myc/TGF? transgenic male mice aged 20?34
weeks were analyzed. On gadoxetate acid-enhanced MRI, the signal intensity was 752.4 for liver parenchyma and
924.5 for HCC. The contrast to noise ratio was 20.4, the percentage enhancement was 267.1% for normal liver
parenchyma and 353.9% for HCC. The fat content was 11.2% for liver parenchyma and 16.2% for HCC. There was
a correlation between fat content and signal intensity with r¼0.7791. All parameters were statistically significant
with P50.05. Our data indicate that gadoxetate acid contrast enhancement allows sensitive detection of HCC
in c-myc/TGF? transgenic mice and determination of the fat content seems to be an additional useful parameter
Keywords: MRI; hepatocellular carcinoma; gadoxetate acid; c-myc/TGF? transgenic mouse model; fat content.
Hepatocellular carcinoma (HCC) is a malignant disease
of the liver and one of the most common malignancies
worldwide. HCC, has a poor prognosis and develops
based on steatohepatitic and cirrhotic predamaged liver
parenchyma. The poor prognosis of HCC is a conse-
quence of the detection of HCC at advanced stages, at
which curative options such as transplantation, surgical
resection or local ablation are not available. Therefore in
the last few years new approaches were developed to
improve early detection of HCC and to improve
This paper is available online at http://www.cancerimaging.org. In the event of a change in the URL address, please use the DOI
provided to locate the paper.
? 2012 International Cancer Imaging Society
monitoring therapeutic success. Appropriately designed
mouse models would be highly useful to facilitate
the development of new tumour diagnostics and thera-
pies. However, neither cell-based assays nor xenograft
models are particularly successful in predicting drug
responses in humans. Genetically engineered mouse
models recapitulating specific pathway abnormalities
such as double transgenic c-myc/TGF? mice might be
more successful at predicting clinical response[2,3]. A
major drawback of the tumour models is the difficulty
to visualize the endogenously formed tumours. Optimal
imaging procedure should be brief and minimally inva-
sive. Magnetic resonance imaging (MRI) satisfies these
criteria and gadoxetate acid (Primovist, Bayer Schering
Pharma AG, Berlin, Germany)-enhanced MRI improves
the detection of HCC[4,5]and has a better performance
than computed tomography or unenhanced MRIand
Gadoxetate acid (gadolinium ethoxybenzyl diethylene-
triamine pentaacetic acid) is a liver-specific lipophilic
contrast agent, behaving both as an extracellular and
hepatobiliary agent. Most malignant lesions including
HCCs and metastatic liver tumours do not take up gadox-
etate acid, resulting in detection of tumours as hypoin-
tense nodules on the hepatobiliary phase of gadoxetate
acid-enhanced MRI. Accordingly, 92% of human HCCs
appear as hypointense nodules in gadoxetate acid-
enhanced MRI, but 3% show a hyperintense signal due
to the overexpression of a particular sodium independent
organic anion transporter. Changes in the fatty com-
ponent are one prognostic marker. Fat content is stated
to be an additional tool to help assess tumour responses
and to determine treatment success or failure of radio-
frequency ablation. To verify a correlation between
the signal intensity of gadoxetate acid-enhanced MRI
and the fat content of HCC, the fat content of HCC
was specified by Dixon in-phase (IP) and opposed-
phase (OP) MRI, confirmed by histopathologic analysis
and compared with the signal intensity of gadoxetate
acid-enhanced T1 weighted fat-suppressed MRI.
The aim of this study was to evaluate gadoxetate acid-
enhanced MRI in a c-myc/TGF? transgenic mouse
model for detecting HCC by determining the relationship
between the signal intensity and the fat content of HCC.
Materials and methods
Twenty-one c-myc/TGF? transgenic mice aged 20?34
weeks were examined by gadoxetate acid-enhanced
MRI. These double transgenic male c-myc/TGF? mice
were generated by breeding the homozygous single trans-
genic mice. Hepatocarcinogenesis was induced by zinc in
the drinking water, resulting in 100% incidence of HCC
after 6?9 months[11,12]. These animals show an overex-
pression of the oncogene c-myc and of transforming
growth factor-alpha (TGF-?). The process was confirmed
by pathologic examination after MRI analysis. The
c-myc/TGF? transgenic mouse model was established
by Thorgeirsson et al. (National Cancer Institute, NIH,
Bethesda, MD, USA) to investigate the molecular events
underlying human hepatic malignant transformation. The
governmental committee and our institutional animal
research review board approved this study.
MRI was performed by 3-T MRI (Siemens Magnetom
Trio, Erlangen, Germany). An MRI coil for 3-T (8 chan-
nel multifunctional coil; NORAS MRI Products GmbH,
H€ ochberg, Germany) was used to increase the signal.
Each coil was configured as a 4-channel array. All
images were obtained in the axial plane. The imaging
sequences included T1-weighted turbo-spin echo with
the following parameters: echo time (TE)¼20ms,
repetition time (TR)¼947ms, field of view¼100mm,
slice thickness¼1mm, flip angle¼140?and fat sup-
pression¼fat saturation. The imaging sequences for
determination of fat content were: IP: TR¼5.96ms,
TR¼5.96ms, TE¼3.675ms, slice thickness¼1mm.
The spatial resolution (matrix) was 160?160 with a
voxel size of 1.2?1.2?1mm.
Mice were anesthetized by intraperitoneal administra-
tion of ketamine and xylazine. Then 200ml of 10mM
gadoxetate acid were injected into the retrobulbar
venous plexus before MRI. The imaging sequences for
determination of fat content were performed immediately
after administration of contrast agent. The sequences to
determine the signal intensity of liver parenchyma and
HCC were obtained before and 20min after administra-
tion of contrast medium.
All MRI examinations were transferred to a picture
archiving and communication system (PACS; Centricity,
Chicago, IL, USA) viewing station. Each lesion was eval-
uated by statistical analysis based on the measurement of
the signal intensity of T1-weighted MRI by operator-
defined regions of interest (ROI). The signal intensity
of liver and HCC were determined. Five ROIs were
selected in normal liver parenchyma, avoiding blood ves-
sels, and 5 ROIs were selected in HCC. The contrast to
noise ratio and percentage enhancement were calculated
based on these values (Table 1). They were expressed as
means?standard deviation (SD).
To assess one normally distributed, independent sample,
the one-sample t test was used. In the case of 2 non-nor-
mally distributed independent samples, the non-para-
metric Mann-Whitney test was used. Results were
considered to be significant at P50.05. Linear regression
Gadoxetate acid-enhanced MRI of hepatocellular carcinoma 73
was determined by single linear regression (Pearson) and
the analysis of matched pairs was performed using the
Wilcoxon matched pairs test.
Determination of fat content
Fat content in MRI was calculated using a formula in
Dixon T1 IP and OP MRI sequences, as shown in Figs. 1
and 2. Five ROIs on each gadoxetate acid-enhanced
image were selected on IP and OP sequences in normal
liver tissue as well as in HCC. They were expressed as
means?SD. The fat content was estimated applying the
Fat content liver
¼(liver signal intensity IP?liver signal intensity OP)
liver signal intensity IP
The resected liver specimens were fixed in 4% formalin
and cut in their entirety in 3-mm slices to facilitate careful
gross examination. Specimens were embedded in paraf-
fin, cut in 4-mm sections, and stained with hematoxylin
and eosin for histopathologic evaluation.
Twenty-one c-myc/TGF? transgenic mice with HCC
were used to detect HCC by gadoxetate acid-enhanced
MRI. One mouse was excluded from this study because
T1 IP and OP images could not be analyzed for the
determination of fat content.
Gadoxetate acid-enhanced MRI parameters
T1-weighted MRI of the liver in a c-myc/ TGF? mouse
with HCC is shown in Fig. 3. On gadoxetate acid admin-
istration, 20 HCC appeared hyperintense in T1-weighted
fat-suppressed MRI. The signal intensity is statistically
images and the comparison between the signal intensity
of the liver and HCC was statistically significant
(P50.05) (Table 2).
The difference between the signal intensity of the
tumour and the liver parenchyma in relation to the back-
ground noise was 20.4?8.3 (P50.05).
Calculation of the statistical parameters of gadoxetate acid-enhanced MRI
Contrast to noise ratio
(Signal intensity of HCC ? signal intensity of liver parenchyma)/SD image noise
[(Signal intensity of Gd-EOB-DTPA-enhanced ? signal intensity of unenhanced)/signal
intensity if unenhanced]?100
Formulas to calculate the contrast to noise ratio and percentage enhancement for image analysis of gadoxetate acid-enhanced MRI of c-myc/TGF?
transgenic mice using the signal intensity and standard deviation (SD) of normal liver parenchyma and HCC.
TGF? mouse. IP chemical shift MR images of the same
lesion (arrow) as in Fig. 3.
Gadoxetate acid-enhanced MRI of a c-myc/
TGF? mouse. OP chemical shift MR images of the
same lesion (arrow) as in Fig. 3.
Gadoxetate acid-enhanced MRI of a c-myc/
74H. Korkusuz et al.
The percentage enhancement of unenhanced com-
pared with gadoxetate acid-enhanced liver parenchyma
(Table 2). This comparison was statistically significant
(P50.05). The comparison between the percentage
enhancement values of HCC and liver parenchyma
revealed that gadoxetate acid enhancement of HCC
was 84.3?35.4% higher than that of normal liver
Correlation of fat content and signal
All mice had steatosis and fatty HCC. The mean value of
the fat content based on McPherson?s formula was
11.2?1.5% for liver parenchyma and 16.2?2.7% for
HCC. According to these values, the difference between
the fat content in liver parenchyma and HCC was statis-
tically significant with P50.05 (Table 2). There was a
correlation between fat content and signal intensity of
r¼0.7791 (Pearson) as shown in Fig. 4. The comparison
of these matched pairs was statistically significant
The pathologic diagnosis and evaluation of the fat con-
tent was made based on resected livers post mortem.
Histologic patterns were primarily well-differentiated
HCCs with a Dixon score of 2 and a fat content of
5?25%, representing mild fatty degeneration (Fig. 5).
Previous studies have shown that MRI is highly useful for
the diagnosis of hepatic lesions. In particular, gadoxetate
acid considerably improved the detection of HCC[14?18].
Other studies have shown that T1-weighted fat-sup-
pressed images are superior to other imaging techniques
such as non-fat-suppressed T1-weighted or T2-weighted
The results of this study confirm that gadoxetate acid is
useful to detect HCC in the c-myc/TGF? transgenic
mouse model. This is shown by the signal intensity, con-
trast to noise ratio and percentage enhancement. HCCs
could be easily distinguished from normal liver parench-
yma as demonstrated by the statistically significant
difference in the signal intensity of normal liver
Statistical parameters of gadoxetate acid-enhanced MRI
HCC Liver parenchymaMean difference:
normal liver vs HCC
Contrast to noise ratio
Fat content (%)
**One-sample t test.
***Wilcoxon matched pairs test.
gadoxetate acid-enhanced liver parenchyma (triangle) and
gadoxetate acid-enhanced HCC (cross) with r¼0.7791
(Pearson). The comparison of these matched pairs was
statistically significant (P50.05) (Wilcoxon matched
Correlation of fat content and signal intensity of
TGF? mouse. T1-weighted 3-T MRI with HCC (arrow)
with hyperintensive enhancement.
Gadoxetate acid-enhanced MRI of a c-myc/
Gadoxetate acid-enhanced MRI of hepatocellular carcinoma 75
parenchyma compared with HCCs. Even more important
for the evaluation of the relation between normal liver
parenchyma and HCC of gadoxetate acid enhancement
is the contrast to noise ratio, which confirmed the differ-
ence in signal intensity between HCC and liver parench-
yma and excluded the influence of the image noise.
Percentage enhancement also verified the benefit of
gadoxetate acid-enhanced MRI in that HCCs showed
an increased uptake of gadoxetate acid compared with
normal liver parenchyma by 84.3?35.4%. In addition,
fatty HCCs are associated with a high signal intensity.
This reflects that HCCs contain a higher fat content than
liver parenchyma. This was confirmed by histology and a
higher signal intensity than the surrounding normal liver
parenchyma. On the other hand, normal liver tissue has a
low fat content and according to this, a low signal inten-
sity. In conclusion, a high percentage enhancement is
associated by a high fat content.
Reason for hyperintensive gadoxetate acid
enhancement: hepatic gadoxetate
Other studies addressing the reason for gadoxetate
acid enhancement of HCC in MRI stated that the
grade of cell differentiation[20,21], bile production, or
necrosis of HCCdid not correlate with the enhance-
ment ratio of gadoxetate acid. The enhancement ratios
correlate with lesion size, bile accumulation in
tumoursand with levels of sodium independent
uptake of gadoxetate acid is considered to represent
passive diffusion mediated by organic anion transporter
polypeptide 1 (OATP1), which is expressed on the hepa-
tocyte membrane. According to this hypothesis, HCC
appear hyperintensive in gadoxetate acid-enhanced MRI
because of the overexpression of transporters such as
OATP1B1, OATP1B3 and NTCP[24?27].
Gadoxetate acid characteristics
After the uptake of gadoxetate acid, the lipophilic con-
trast agent remains in the hepatocytes. Our study shows a
correlation between gadoxetate acid-enhanced signal
intensity and fat content (r¼0.7791), which is visualized
in Fig. 4. HCCs often contain an adipose component and
show various grades of fatty infiltration, which may be
diffuse throughout the tumour, in focal areas or in a
patchy pattern. The fat content was even more frequent
in small HCC nodules[10,28]. The mean value of fat con-
tent was 16.2% for HCCs and 11.2% for normal liver
parenchyma in c-myc/TGF? mice. This suggests that
the metabolic activity is reprogrammed towards steatosis.
This internal fat deposition is assessed by quantification
of intrahepatocellular lipid by MRI by exploiting charac-
teristic differences in resonant frequencies between pro-
tons in fat and water environments, determining the
differences in intensity between IP and OP images[29?31].
MRI is proven to be comparably accurate in quantifying
hepatic fat contentand neither interaction between
Dixon IP/OP or?fat saturation and stage of fibrosis or
hepatic inflammation affect the accuracy of MRI for the
assessment of steatosis. Therefore, MRI is an elegant
method for the non-invasive quantification of the hepatic
Increased lysophosphatidic acid (LPA) may provide
a potent mitogenic and proliferative microenvironment
via autocrine and paracrine activation of high-affinity
G-protein-coupled receptors and cellular proliferation is
accompanied by reprogramming of metabolic activity,
such as high rates of glycolysis, lactate production and
lipid biosynthesis. Hence lipids are linked to pathologic
process such as inflammation, obesity and liver disease,
and they are involved in cellular signaling. They are
also involved in apoptosis and cell cycle regulation.
Specific receptors, Rho and Rho kinase, have the ability
to stimulate cell proliferation. For that reason, LPA sig-
naling has been linked to cancer, which means that an
overexpression of Rho-GTPase binding proteins is asso-
ciated with lysophosphatidic acid signaling. The current
hypothesis is that control of metabolic activity in tumour
cells is synchronous with that of growth factor signaling.
The fat content in HCC and in liver parenchyma of
c-myc/TGF? transgenic mice make it likely that growth
factor signaling regulates metabolic activity. This may
explain the high fat content of HCC compared with
normal liver parenchyma. Thus, the increased fat content
of HCC may retain gadoxetate acid in HCC cells, pre-
venting hepatic secretion via the multidrug-resistance
associated proteins, ABCB4 and ABCC2.
Fat content as a prognostic marker
of successful therapy
Fat content is a valuable predictor of successful therapy.
This is demonstrated by other studies. They stated that
(arrow) with a Dixon score of 2.
Normal liver parenchyma and liver tumour
76 H. Korkusuz et al.
visceral fat accumulation, which is related to the severity
of fatty liver, increases the risk of HCC development and
is an independent risk factor of HCC after curative treat-
ment. In particular, a high fat content of the liver,
with regard to non-alcoholic steatohepatitis, is a risk
factor for HCC[36?38]. Other risk factors are age 462
years, poor histopathologic grading, multifocal tumour,
portal vein thrombosis, higher alpha-fetoprotein and
serum bilirubin levels[38,39]. The risk factor fat content
can be surveyed by gadoxetate acid-enhanced MRI. In
accordance with our results that fat content and signal
intensity correlate, high signal intensity by gadoxetate
acid-enhanced MRI might be used as a prognostic
marker of successful therapy. According to this, changes
in the fatty component might be used to monitor therapy.
Thus, Pupulim et al.demonstrated monitoring for
therapy success of radiofrequency ablation.
Benefit of gadoxetate acid-enhanced MRI
in the c-myc/TGF? mouse model
Transgenic HCC mouse models such as the c-myc/TGF?
model used in the present study are valuable for detecting
HCC due to its similarity to human HCC. The advantage
of mouse models in research has already been shown by
the detection of HCC initiation and progression in trans-
genic mouse models such as alb-myctg with clinical 1.5-T
MR scanners and gadoxetate acid enhancement by
revealing hypointense lesions.
The second advantage of gadoxetate acid-enhanced
MRI for detecting HCC is the relation between signal
intensity and fat content. Our study indicates that one
reason for gadoxetate acid enhancement is steatosis.
Therefore, changes in the fatty component could be an
additional finding to help to assess tumour responses and
success of therapy. This was revealed by a study of radio-
frequency ablation of fatty HCCs and suggests that fat
content could be used as a prognostic marker for HCC
Even though this transgenic mouse model for detecting
HCC has many advantages, such as histologic similarity
to human tumours, the tumours arise in immunocompe-
tent mice, metastatic distribution similar to the clinical
situation, relevant host immune cell infiltration and
tumour microenvironment, the results of gadoxetate
acid enhancement cannot uncritically be transferred to
Highly hepatocyte-selective enhancement of gadoxetate
acid is correlated with the amount of HCC fat content.
Gadoxetate acid enhancement, based on its correlation
with the fat content, might be a useful tool as a prognos-
tic marker or for monitoring therapy. This animal model
may help to develop a better understanding of HCC
gadoxetate acid contrast enhancement and thus of
HCC diagnostics and therapy.
We thank Snorri S. Thorgeirsson (National Cancer
Institute, NIH, Bethesda, MD, USA) for kindly providing
c-myc/TGF? transgenic mice.
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