Recent trends in antibody based sensors
ABSTRACT This review details recent advances in the fields of immunosensors and closely related immunoassays in the past decade, together with a discussion of possible future trends. Immunosensors can be classified by the way in which they transduce the signal produced upon the formation of an antibody antigen complex. Recent advancements to these methods of detection and transduction are discussed in detail, with particular focus on electrochemical, optical, piezoelectric and magnetic based sensors. The varying applications of these sensors are also discussed. Some of the most significant advances include development of immunosensors for the continuous monitoring of analytes, point of care (PoC) devices, with lower unit costs, automation, reusability and ease of use. Immunosensor technology has advanced at a prolific rate since its conception and has grown into a diverse area of ongoing research.
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ABSTRACT: A protocol for the creation of a set of classification models was developed to differentiate between biological samples based on immunosensor measurements. For this paper, data was gathered using Au Quartz Crystal Microbalance with Dissipation (QCM-D) sensors inoculated with an alkanethiol self-assembling monolayer functionalized for the detection of pAkt, gamma H2AX, beta-Actin, and FITC antigen expression. Oropharyngeal cancer lysate samples, both positive (SCC47) and negative (TU212) for high risk human papillomavirus (HPV16), were used to gather the classification model training data set. Subsequently, linear and Bayesian classifiers were formulated based on the feature values and defined linear discriminant functions. The following study distinguishes between HPV-positive and HPV-negative cell lines, yet these guidelines can be utilized for different immunosensor platforms and disease diagnosis.Sensors and Actuators B Chemical 01/2014; 190:165-170. DOI:10.1016/j.snb.2013.08.081 · 3.84 Impact Factor
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ABSTRACT: This study described the synthesis and characterization of a multi-wall carbon nanotube/poly(amidoamine) dendrimer (MWCNT-PAMAM) hybrid material and its application in the fabrication of piezoelectric immunosensing platform. The aim of modifying PAMAM dendrimers onto the MWCNT surface was to improve its antibody adsorption and dispersion in aqueous phase, which was contributed to enhance the sensitivity and stability in piezoelectric immunoassay procedure. The adsorption performance and properties of the MWCNT-PAMAM hybrid were investigated using the Langmuir and Freundlich isotherm models, and possible mechanisms were discussed. The maximum antibody adsorption capacity was estimated as 147.06 mu g mu g(-1), which is higher than any value currently reported for MWCNT. Based on these results, we developed a novel MWCNT-PAMAM-based piezoelectric immunosensing platform, which offered a stable, sensitive and accurate immunoassay for the determination of amounts of a carbamate pesticide-metolcarb, resulting in a linear range of 0.1-50.0 mg L-1 with detection limit of 0.019 mg L-1. And the mentioned immunosensing platform also exhibited satisfactory application capability in spiked apple and orange juice samples with recoveries of 81.9-101.2% and a standard deviation of 2.1-6.8% (n = 7).Sensors and Actuators B Chemical 11/2013; 188:949-956. DOI:10.1016/j.snb.2013.07.116 · 3.84 Impact Factor
Future Virology 06/2014; 9(6):595-603. DOI:10.2217/fvl.14.33 · 1.00 Impact Factor