Isolation and molecular characterization of Candida africana from Jos, Nigeria
ABSTRACT During a survey of the prevalence of Candida spp. in Jos, Plateau State, Nigeria, two atypical C. albicans isolates were recovered. These two yeasts were germ tube positive, chlamydospore-negative and gave a green color on CHROMagar Candida. Molecular analysis performed by amplification of the hwp1 gene showed that these two isolates belonged to C. africana, a newly proposed Candida species closely related to C. albicans. Based on the presence or absence of an intron in DNA sequences encoding rRNA, the two C. africana, including all C. albicans isolates examined, were found to belong to genotype A and no other genotypes or species such as C. dubliniensis were found. To our knowledge, this is the first isolation of C. africana in Nigeria.
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Article: Isolation and molecular characterization of Candida africana from Jos, Nigeria
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- "Currently, only few molecular methods have been reported for specific identification of C. africana (Table 2). One of these, has been widely used in several epidemiological studies (Albaina et al., 2015; Gumral et al., 2011; Ngouana et al., 2015; Nnadi et al., 2012; Romeo and Criseo, 2009b; Shan et al., 2014; Sharma et al., 2014) and it is a simple PCR-based method that uses only a single pair of primer specifically designed to span a highly polymorphic region within the hyphal wall protein 1 (HWP1) gene (Romeo and Criseo, 2008). This method allows one to identify all members of the C. albicans complex in a single PCR reaction because it produces different size amplicons (C. "
ABSTRACT: In recent years, the taxonomy of the most important pathogenic Candida species (Candida albicans, Candida parapsilosis and Candida glabrata) has undergone profound changes due to the description of new closely-related species. This has resulted in the establishment of cryptic species complexes difficult to recognize in clinical diagnostic laboratories. The identification of these novel Candida species seems to be clinically relevant because it is likely that they differ in virulence and drug resistance. Nevertheless, current phenotypic methods are not suitable to accurately distinguish all the species belonging to a specific cryptic complex and therefore their recognition still requires molecular methods. Since traditional mycological techniques have not been useful, a number of molecular based methods have recently been developed. These range from simple PCR-based methods to more sophisticated real-time PCR and/or MALDI-TOF methods. In this article, we review the current methods designed for discriminating among closely related Candida species by highlighting, in particular, the limits of the existing phenotypic tests and the development of rapid and specific molecular tools for their proper identification. Copyright © 2015 Elsevier B.V. All rights reserved.Journal of Microbiological Methods 02/2015; 111C:50-56. DOI:10.1016/j.mimet.2015.02.004 · 2.10 Impact Factor
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- "No C. dubliniensis, C. inconspicua, and D. hansenii isolates were derived from DGP ICUs (Table 2) and none of these strains were found in DGP-specimens, except DGP swabs (Table 2). Gumral et al.  reported the lack of C. dubliniensis and C. africana strains in Turkey with vaginal C. albicans isolates, whilst Nnadi et al.  found in Nigeria no C. dubliniensis in vulvovaginal samples. As three C. africana isolates appeared in Berlin and two in Munich they were tested during a MCS in 2000, together with the strains from Angola and Madagascar. "
ABSTRACT: From 1997 to 2009, 1,862 dermatology, gynaecology, and paediatrics (DGP) associated clinical yeast isolates were analysed for species occurrence, specimen origin and type, (multi-) resistance pattern, and testing period. The top seven of the isolated DGP-associated species remained the same as compared to total medical wards, with Candida albicans (45%) as most frequent pathogen. However, the DGP wards and DGP ICUs showed species-specific profiles; that is, the species distribution is clinic-specific similar and however differs in their percentage from ward to ward. By applying the "one fungus one name" principle, respectively, the appropriate current taxonomic species denominations, it has been shown that no trend to emerging species from 1998 to 2008 could be detected. In particular the frequently isolated non-Candida albicans species isolated in the DGP departments have already been detected in or before 1997. As yeasts are part of the cutaneous microbiota and play an important role as opportunistic pathogens for superficial infections, proper identification of the isolates according to the new nomenclature deems to be essential for specific and calculated antifungal therapy for yeast-like DGP-related infectious agents.International Journal of Microbiology 12/2013; 2013:703905. DOI:10.1155/2013/703905
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ABSTRACT: Candida africana was previously proposed as a new species within the Candida albicans species complex, together with C. albicans and C. dubliniensis, although further phylogenetic analyses better support its status as an unusual variant within C. albicans. Here we show that C. africana can be distinguished from C. albicans and C. dubliniensis by Pyrosequencing® of a short region of ITS2, and have evaluated its occurrence in clinical samples by Pyrosequencing® all presumptive isolates of C. albicans submitted to the Mycology Reference Laboratory over a 9 month period. The C. albicans complex constituted 826/1839 (44.9%) of yeast isolates received over the study period and included 783 isolates of C. albicans, 28 isolates of C. dubliniensis and 15 isolates of C. africana. In agreement with previous reports, C. africana was isolated exclusively from genital specimens, in women in the 18-35 years age group. Indeed, C. africana constituted 15/251 (6%) of "C. albicans" isolates from female genital specimens during the study period. C. africana isolates were germ-tube positive, grew significantly more slowly than C. albicans and C. dubliniensis on conventional mycological media, could be distinguished from the other members of the C. albicans complex by appearance on chromogenic agar, and were incapable of forming chlamydospores. Here we present the detailed evaluation of epidemiological, phenotypic and clinical features and antifungal susceptibility profiles of UK isolates of C. africana. Furthermore, we demonstrate that C. africana is significantly less pathogenic than C. albicans and C. dubliniensis in the Galleria mellonella insect systemic infection model.Journal of clinical microbiology 01/2013; 51(3). DOI:10.1128/JCM.02816-12 · 4.23 Impact Factor