Mass spectral measurements of the apoHDL in horse (Equus caballus) cerebrospinal fluid

The Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA.
Comparative Biochemistry and Physiology Part D Genomics and Proteomics (Impact Factor: 2.06). 02/2012; 7(2):172-4. DOI: 10.1016/j.cbd.2012.02.002
Source: PubMed


As a continuation of our proteogenomic studies of equine apolipoproteins, we have obtained molecular masses for several of the apolipoproteins associated with the HDL in horse cerebrospinal fluid (CSF). Using electrospray-ionization mass spectrometry (ESI-MS), we report on values for apolipoproteins, A-I and A-II, as well as acylated apoA-I. In comparison with our previously published data on equine plasma apolipoproteins, there appears to be a higher percentage of acylated apoA-I in the CSF than in plasma. As was the case in plasma, apoA-II circulates as a homodimer. These studies also revealed a protein with a mass of 34,468Da that we are speculating is the value for horse apoE.

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