Reproducibility of mass spectrometry based protein profiles for diagnosis of ovarian cancer across clinical studies: A systematic review

Institute of Regional Health Services Research, University of Southern Denmark, Odense, Denmark.
Journal of proteomics (Impact Factor: 3.89). 02/2012; 75(10):2758-72. DOI: 10.1016/j.jprot.2012.02.007
Source: PubMed


The focus of this systematic review is to give an overview of the current status of clinical protein profiling studies using MALDI and SELDI MS platforms in the search for ovarian cancer biomarkers. A total of 34 profiling studies were qualified for inclusion in the review. Comparative analysis of published discriminatory peaks to peaks found in an original MALDI MS protein profiling study was made to address the key question of reproducibility across studies. An overlap was found despite substantial heterogeneity between studies relating to study design, biological material, pre-analytical treatment, and data analysis. About 47% of the peaks reported to be associated to ovarian cancer were also represented in our experimental study, and 34% of these redetected peaks also showed a significant difference between cases and controls in our study. Thus, despite known problems related to reproducibility an overlap in peaks between clinical studies was demonstrated, which indicate convergence toward a set of common discriminating, reproducible peaks for ovarian cancer. The potential of the discriminating protein peaks for clinical use as ovarian cancer biomarkers will be discussed and evaluated. This article is part of a Special Issue entitled: Proteomics: The clinical link.

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    • "Of all the proteomic approaches used for this purpose , peptide and protein profiling has become a technique very close to clinical application [9]. Peptide/protein profiling is based on the detection of discriminatory patterns that enable classifying and distinguishing between different study populations (e.g., patients versus healthy controls) and is currently being used as a clinical tool for the diagnosis of ovarian cancer [9], microbial typing [10] or MS imaging [11]. The peptide/protein pattern is comprised of combinations of peaks in the mass spectra plot that are defined by the intensity of each signal on the y-axis, and the mass-to-charge ratio on the xaxis . "
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    EuPA Open Proteomics 06/2014; 3. DOI:10.1016/j.euprot.2014.02.016
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    • "They are, however, not very specific, although different insults may produce different patterns of acute phase response (APR). Many of the reported diagnostic SELDI peaks have been found to be acute phase proteins, and are described in several reviews [11,19,37,38]. SAA is primarily induced by pro-inflammatory cytokines such as IL-1β, TNF-α and IL-6, which are released by a variety of cells including activated tissue macrophages and blood monocytes in response to injury [39,40]. "
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    BMC Research Notes 11/2013; 6(1):466. DOI:10.1186/1756-0500-6-466
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    • "A large number of review articles have appeared in the past several years, offering excellent overviews and perspectives on novel proteomic applications in cancer. Many reviews focused on different cancer types, such as breast cancer,1–4 pancreatic cancer,5,6 ovarian cancer,7–9 colorectal cancer,10,11 and glioma.12–14 Others have focused on sample types or subcellular components, such as tissue,15–17 serum,18–20 and secretome.21–23 "
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