"Full list of author information is available at the end of the article isolation and breeding of high-producing strains, the utilization of inducers, and the heterologous expression of Lcc genes (Wang et al. 2013). Furthermore, most of Lcc are extracellular inducible enzymes, their rates of synthesis and activity being strongly dependent on the presence of suitable inductor which plays an important role in increasing their production (Kocyigit et al. 2012). "
[Show abstract][Hide abstract] ABSTRACT: The capability of the fungi Nigrospora sp. CBMAI 1328 and Arthopyrenia sp. CBMAI 1330 isolated from marine sponge to synthesise laccases (Lcc) in the presence of the inducer copper (1–10 μM) was assessed. In a liquid culture medium supplemented with 5 μM of copper sulphate after 5 days of incubation, Nigrospora sp. presented the highest Lcc activity (25.2 U·L−1). The effect of copper on Lcc gene expression was evaluated by reverse transcriptase polymerase chain reaction. Nigrospora sp. showed the highest gene expression of Lcc under the same conditions of Lcc synthesis. The highest Lcc expression by the Arthopyrenia sp. was detected at 96 h of incubation in absence of copper. Molecular approaches allowed the detection of Lcc isozymes and suggest the presence of at least two undescribed putative genes. Additionally, Lcc sequences from the both fungal strains clustered with other Lcc sequences from other fungi that inhabit marine environments.
AMB Express 03/2015; 5(1):19. DOI:10.1186/s13568-015-0106-7
"udied habitats , yeast isolates were tested for their ability to secrete extracellular hydrolases ( amylase , esterase , chiti - nase , DNAse , pectinase , protease , and urease ) and redox en - zymes ( laccase ) at 4 and 20 C , according to the protocols previously described ( de Garc ıa et al . 2007 ; Brizzio et al . 2007 ; Branda et al . 2010 ; Kocyigit et al . 2012 ) . Fig 2 e Maximum Likelihood ( ML ) phylogenic tree of strains belonging to the class Tremellomycetes generated on the base of D1 / D2 optimized alignment . The tree , based on 82 sequences and 643 nucleotide positions , was generated using GTR D G ( 4 ) model calculated using ML in MrAIC software . Base frequencies were as follows : "
[Show abstract][Hide abstract] ABSTRACT: Yeast strains isolated from rock samples collected from worldwide cold regions were identified by sequence analysis of the D1/D2 domains of the 26S rDNA gene and the ITS region followed by molecular phylogeny. Over 77 % of yeasts isolates were Basidiomycota. Cryptococcus (orders Filobasidiales and Tremellales) and Rhodotorula (order Cystobasidiales) were the most frequent genera. About 40 % of yeast isolates belonged to undescribed species. Almost all isolates were psychrotolerant. Urease and esterase were the most widely extracellular enzymatic activity at 4 °C and 20 °C. None of the strains exhibited extracellular protease, DNAse, cellulase, chitinase, and laccase activity. The taxonomic and ecological significance of yeasts associated to worldwide cold rocky habitats is discussed.
[Show abstract][Hide abstract] ABSTRACT: A basidiomycete strain MT showing lignin degradation capability was isolated and identified as a Trametes trogii strain, based on the morphological characteristics and the ITS 5.8S sequence. Enzyme production by the organism under liquid fermentation and during the decay process of a 120-day incubation period on Populus wood was studied. The results showed that the strain can produce high activities of laccase and manganese peroxidases, but no lignin peroxidases, and has the ability to simultaneously degrade lignin, cellulose, as well as hemicellulose of poplar wood. The decay pattern and process were explored using scanning electron microscopy and infrared spectroscopy. The results showed that the poplar wood is decomposed from the lumen to the middle lamellae, all the wood cell wall components are degraded, and the lignin and carbohydrate decay with no obvious differences in the levels of degradation. The decay patterns of MT in poplar wood showed the simultaneous type characteristics. The secretome of MT was surveyed using a shotgun strategy, and 65 proteins were assigned unambiguously, including the proteins with the functions of carbohydrate metabolism, cell wall and lignin degradation, fatty acid metabolism, protein metabolism and other functions. The secretome data showed that the fungus might have a complex enzymatic system implicated in lignocellulose degradation. The information presented in this paper is helpful to better understand the lignocellulose degradation mechanisms of T. trogii strains.
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