Advances in Wound-Healing Assays for Probing Collective Cell Migration

Department of Aerospace and Mechanical Engineering, The University of Arizona, Tucson, AZ, USA.
Journal of the Association for Laboratory Automation (Impact Factor: 1.5). 02/2012; 17(1):59-65. DOI: 10.1177/2211068211426550
Source: PubMed

ABSTRACT Collective cell migration plays essential roles in a wide spectrum of biological processes, such as embryogenesis, tissue regeneration, and cancer metastasis. Numerous wound-healing assays based on mechanical, chemical, optical, and electrical approaches have been developed to create model "wounds" in cell monolayers to study the collective cell migration processes. These approaches can result in different microenvironments for cells to migrate and possess diverse assay characteristics in terms of simplicity, throughput, reproducibility, and multiplexability. In this review, we provide an overview of advances in wound-healing assays and discuss their advantages and limitations in studying collective cell migration.

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Available from: Reza Riahi, May 10, 2014
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