Advances in Wound-Healing Assays for Probing Collective Cell Migration

Department of Aerospace and Mechanical Engineering, The University of Arizona, Tucson, AZ, USA.
Journal of the Association for Laboratory Automation (Impact Factor: 1.5). 02/2012; 17(1):59-65. DOI: 10.1177/2211068211426550
Source: PubMed

ABSTRACT Collective cell migration plays essential roles in a wide spectrum of biological processes, such as embryogenesis, tissue regeneration, and cancer metastasis. Numerous wound-healing assays based on mechanical, chemical, optical, and electrical approaches have been developed to create model "wounds" in cell monolayers to study the collective cell migration processes. These approaches can result in different microenvironments for cells to migrate and possess diverse assay characteristics in terms of simplicity, throughput, reproducibility, and multiplexability. In this review, we provide an overview of advances in wound-healing assays and discuss their advantages and limitations in studying collective cell migration.

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Available from: Reza Riahi, May 10, 2014
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    • "Compared with simple proliferation assays, migration assays add another dimension and allow easy screening of many compounds. Wound healing assays, where confluent monolayers of ECs are mechanically or chemically wounded in a standardised manner, combine proliferation and migration and also allow easy screening end evaluation of the effects of many compounds [28]. "
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    • "In these assays, a cell layer is allowed to move into a cell-free region, and the decrease in time of this area reflects cell migration velocity. The cell-free zone can be created (Riahi et al., 2012) by scratching a confluent cell layer [typical wound healing assay (Liang et al., 2007)] or by using non-damaging barriers during cell seeding [cell exclusion zone assay (Gough et al., 2011)]. Advanced microscopes then allow automatic acquisition of images of the migrating cells, and specialized image processing tools are used to quantify cell migration features from these images (Table 1). "
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