Genome Sequence of Paenibacillus terrae HPL-003, a Xylanase-Producing
Bacterium Isolated from Soil Found in Forest Residue
Sang Heum Shin,aSewhan Kim,aJae Young Kim,aHa Young Song,bSoo Jin Cho,bDal Rye Kim,bKee-In Lee,bHee Kyung Lim,b
No-Joong Park,bIn Taek Hwang,band Kap-Seok Yanga
Macrogen Inc., Gasan-dong, Seoul, Republic of Korea,aand Biorefinery Research Center, Green Chemistry Division, Korea Research Institute of Chemical Technology,
Daejeon, Republic of Koreab
This article reports on the full genome sequence of Paenibacillus terrae HPL-003, which is a Gram-positive, endospore-forming,
important enzyme, as xylanase is an indispensable enzyme in bio-
forest residue on Gara Mountain, is considered the best producer
(4a). This strain was identified as Paenibacillus terrae by 16S rRNA
gene sequencing and physiological analysis (2), showing the highest
16S rRNA gene sequence identity, 99.4%, to strain AM141T(NCBI
accession number AF391124) (10). P. terrae HPL-003 is a Gram-
positive, endospore-forming, xylanase-producing bacterium, and
whole-genome sequencing was carried out in order to facilitate un-
derstanding of the unique properties of P. terrae HPL-003 at the
Genomic DNA isolated from an overnight culture of strain P.
terrae HPL-003 using a DNeasy blood and tissue kit (Qiagen) was
lowing the manufacturer’s instructions, with 25? coverage (5).
Then, the 529,514 reads generated, with a length of 112,887,195
bp, were assembled using a GS De Novo assembler (version 2.3;
Roche), and 506,076 reads (95.57% of the total) were assembled
into 11 scaffolds, with a length of 6,039,623 bp.
The order of scaffolds was determined by a similarity search
among published references and confirmed by a PCR size check.
sequencer. The sequences from ABI 3730 sequencing and scaffolds
The complete genome is composed of a circular chromosome of
6,083,395 bp (46.77% GC content), which includes 5,845 coding
A detailed analysis of a full genome comparison of this and
other available Paenibacillus strains will be included in a future
publication on transcriptome analysis. As the first genome se-
quence study of P. terrae, the present study will provide the basis
for a better understanding of its genetic background, which in
turn will aid future studies.
Nucleotide sequence accession number. The complete ge-
nome sequence of Paenibacillus terrae HPL-003 has been depos-
ited in GenBank under accession number CP003107.
This study was conducted by a research fund supported by the Agricul-
tural R&D Promotion Center (ARPC) in the Republic of Korea (grant
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Received 13 December 2011 Accepted 20 December 2011
Address correspondence to Kap-Seok Yang, email@example.com.
S.H.S. and S.K. contributed equally to this article.
Copyright © 2012, American Society for Microbiology. All Rights Reserved.
jb.asm.org 0021-9193/12/$12.00Journal of Bacteriologyp. 1266