Article

Structure-based search and in vitro analysis of self-cleaving ribozymes.

Department of Pharmaceutical Sciences, University of California, Irvine, CA, USA.
Methods in molecular biology (Clifton, N.J.) 01/2012; 848:131-43. DOI:10.1007/978-1-61779-545-9_9 pp.131-43
Source: PubMed

ABSTRACT Detecting functional RNAs is increasingly accomplished through structure-based searches for patterns of conserved secondary structure. With large amounts of new sequencing data becoming available, there is a greater demand for efficient methods of identifying new RNAs. Here we present a method of identifying self-cleaving ribozymes and characterizing the in vitro activity.

0 0
 · 
0 Bookmarks
 · 
26 Views
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

conserved secondary structure
 
Detecting functional RNAs
 
greater demand
 
large amounts
 
new sequencing data
 
self-cleaving ribozymes
 
structure-based searches
 

Randi M Jimenez