Article

Siliques are Red1 from Arabidopsis acts as a bidirectional amino acid transporter that is crucial for the amino acid homeostasis of siliques.

Zentrum für Molekularbiologie der Pflanzen, Plant Physiology, D-72076 Tuebingen, Germany.
Plant physiology (impact factor: 6.53). 02/2012; 158(4):1643-55. DOI:10.1104/pp.111.192583 pp.1643-55
Source: PubMed

ABSTRACT Many membrane proteins are involved in the transport of nutrients in plants. While the import of amino acids into plant cells is, in principle, well understood, their export has been insufficiently described. Here, we present the identification and characterization of the membrane protein Siliques Are Red1 (SIAR1) from Arabidopsis (Arabidopsis thaliana) that is able to translocate amino acids bidirectionally into as well as out of the cell. Analyses in yeast and oocytes suggest a SIAR1-mediated export of amino acids. In Arabidopsis, SIAR1 localizes to the plasma membrane and is expressed in the vascular tissue, in the pericycle, in stamen, and in the chalazal seed coat of ovules and developing seeds. Mutant alleles of SIAR1 accumulate anthocyanins as a symptom of reduced amino acid content in the early stages of silique development. Our data demonstrate that the SIAR1-mediated export of amino acids plays an important role in organic nitrogen allocation and particularly in amino acid homeostasis in developing siliques.

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    Article: A gene expression map of Arabidopsis thaliana development.
    Markus Schmid, Timothy S Davison, Stefan R Henz, Utz J Pape, Monika Demar, Martin Vingron, Bernhard Schölkopf, Detlef Weigel, Jan U Lohmann
    [show abstract] [hide abstract]
    ABSTRACT: Regulatory regions of plant genes tend to be more compact than those of animal genes, but the complement of transcription factors encoded in plant genomes is as large or larger than that found in those of animals. Plants therefore provide an opportunity to study how transcriptional programs control multicellular development. We analyzed global gene expression during development of the reference plant Arabidopsis thaliana in samples covering many stages, from embryogenesis to senescence, and diverse organs. Here, we provide a first analysis of this data set, which is part of the AtGenExpress expression atlas. We observed that the expression levels of transcription factor genes and signal transduction components are similar to those of metabolic genes. Examining the expression patterns of large gene families, we found that they are often more similar than would be expected by chance, indicating that many gene families have been co-opted for specific developmental processes.
    Nature Genetics 06/2005; 37(5):501-6. · 35.53 Impact Factor
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

able
 
amino acid content
 
amino acid homeostasis
 
amino acids
 
anthocyanins
 
Arabidopsis thaliana
 
chalazal seed coat
 
membrane protein Siliques
 
membrane proteins
 
organic nitrogen allocation
 
plant cells
 
plants
 
plasma membrane
 
seeds
 
SIAR1
 
SIAR1 localizes
 
SIAR1-mediated export
 
siliques
 
translocate amino acids bidirectionally
 
vascular tissue