Chemical synthesis of an erythropoietin glycoform containing a complex-type disialyloligosaccharide.

Department of Chemistry, Graduate School of Science, Osaka University, 1-1, Machikaneyama, Toyonaka, 560-0043 Japan.
Angewandte Chemie International Edition (Impact Factor: 11.34). 02/2012; 51(15):3567-72. DOI: 10.1002/anie.201109034
Source: PubMed

ABSTRACT New and improved: New reaction conditions for tert-Boc-based solid-phase peptide synthesis make acid-labile sialyloligosaccharyl peptide α-thioesters accessible. To demonstrate this, a sialyloligosaccharyl-erythropoietin glycoform with 166 amino acid residues was synthesized.

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