Article

Genetic crosses between Ectocarpus strains.

UPMC Université Paris 06, The Marine Plants and Biomolecules Laboratory, UMR 7139, Station Biologique de Roscoff, BP74, 29682 Roscoff Cedex, France.
Cold Spring Harbor Protocols (impact factor: 4.63). 02/2012; 2012(2):262-5. DOI:10.1101/pdb.prot067942 pp.262-5
Source: PubMed

ABSTRACT This article describes a procedure for conducting crosses between different strains of Ectocarpus. Crossing gametophytes to obtain the sporophyte generation is the most technically challenging stage of this process because diploid sporophytes have to be distinguished from the haploid partheno-sporophytes that result from the parthenogenetic germination of unfused gametes. This requires careful monitoring of the progeny of the genetic cross until they have developed sufficiently to be transferred to a separate Petri dish. Genetic crosses allow several classical genetic methodologies to be applied in Ectocarpus, including allelic complementation tests, backcrosses, combination of different genetic mutations, and outcrosses to create mapping populations.

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Keywords

allelic complementation tests
 
classical genetic methodologies
 
different strains
 
diploid sporophytes
 
distinguished
 
haploid partheno-sporophytes
 
parthenogenetic germination
 
populations
 
requires careful monitoring
 
separate Petri dish