Article

Bioactivity-guided fractionation and analysis of compounds with anti-influenza virus activity from Gardenia jasminoides Ellis.

Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
Archives of Pharmacal Research (impact factor: 1.59). 01/2012; 35(1):9-17. DOI:10.1007/s12272-012-0101-3 pp.9-17
Source: PubMed

ABSTRACT Bioassay-guided fractionation of extracts from Fructus Gardeniae led to analysis of its bioactive natural products. After infection by influenza virus strain A/FM/1/47-MA in vivo, antiviral activity of the extracts were investigated. The target fraction was orally administered to rats and blood was collected. High-performance liquid chromatography coupled with photo diode array detector and electrospray ion trap multiple-stage tandem mass spectrometry was applied to screen the compounds absorbed into the blood. A structural characterization based on the retention time, ultraviolet spectra, parent ions and fragmentation ions was performed. Thirteen compounds were confirmed or tentatively identified. This provides an accurate profile of the composition of bioactive compounds responsible for the anti-influenza properties.

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Keywords

accurate profile
 
anti-influenza properties
 
electrospray ion trap multiple-stage tandem mass spectrometry
 
Fructus Gardeniae
 
High-performance liquid chromatography
 
influenza virus strain A/FM/1/47-MA
 
photo diode array detector
 
structural characterization
 
target fraction
 
ultraviolet spectra