Article

MicroRNA-125b induces cancer cell apoptosis through suppression of Bcl-2 expression.

Department of Obstetrics and Gynecology, General Hospital of PLA, Beijing, China.
Journal of Genetics and Genomics (impact factor: 1.88). 01/2012; 39(1):29-35. DOI:10.1016/j.jgg.2011.12.003 pp.29-35
Source: PubMed

ABSTRACT MicroRNAs (miRNAs) are small, noncoding RNAs which can often act as an oncogene or a tumor suppressor. Several miRNAs are associated with the development of hepatocellular carcinoma (HCC). We demonstrated that miR-125b significantly suppresses HCC cell proliferation and promotes apoptosis by inhibiting the gene expression of the anti-apoptotic protein, Bcl-2. Bioinformatic analysis indicated that the 3'UTR of Bcl-2 has binding sites for miR-125b. Luciferase reporter assay confirmed the ability of miR-125b to dramatically suppress Bcl-2 transcription, suggesting that Bcl-2 is a target gene for miR-125b. We concluded that miR-125b acts as a tumor suppressor in hepatic tumor development by targeting Bcl-2 and inducing cancer cell apoptosis.

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Keywords

anti-apoptotic protein
 
Bcl-2
 
Bcl-2 transcription
 
gene expression
 
HCC
 
hepatic tumor development
 
inducing cancer cell apoptosis
 
Luciferase reporter assay
 
MicroRNAs
 
miR-125b acts
 
miRNAs
 
oncogene
 
promotes apoptosis
 
target gene
 
tumor suppressor