APC/C-mediated multiple monoubiquitination provides an alternative degradation signal for cyclin B1

Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA.
Nature Cell Biology (Impact Factor: 19.68). 02/2012; 14(2):168-76. DOI: 10.1038/ncb2425
Source: PubMed

ABSTRACT The anaphase-promoting complex or cyclosome (APC/C) initiates mitotic exit by ubiquitylating cell-cycle regulators such as cyclin B1 and securin. Lys 48-linked ubiquitin chains represent the canonical signal targeting proteins for degradation by the proteasome, but they are not required for the degradation of cyclin B1. Lys 11-linked ubiquitin chains have been implicated in degradation of APC/C substrates, but the Lys 11-chain-forming E2 UBE2S is not essential for mitotic exit, raising questions about the nature of the ubiquitin signal that targets APC/C substrates for degradation. Here we demonstrate that multiple monoubiquitylation of cyclin B1, catalysed by UBCH10 or UBC4/5, is sufficient to target cyclin B1 for destruction by the proteasome. When the number of ubiquitylatable lysines in cyclin B1 is restricted, Lys 11-linked ubiquitin polymers elaborated by UBE2S become increasingly important. We therefore explain how a substrate that contains multiple ubiquitin acceptor sites confers flexibility in the requirement for particular E2 enzymes in modulating the rate of ubiquitin-dependent proteolysis.

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Available from: Donald S Kirkpatrick, Sep 27, 2015
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    • "It has been reported that K11-polyUb chains formed by the APC/C bind to hHR23B and S5A/Rpn10 to facilitate the degradation of cell-cycle regulators (Jin et al., 2008; Meyer and Rape, 2014), but ambiguity remains regarding which chain types mediated these effects . A key advance of our study is that we used polyUb conjugates formed on a single-lysine residue, thereby preventing the formation of multiple monoUb linkages, which are sufficient to degrade cyclin B1 in Xenopus cell extracts (Dimova et al., 2012). Whether multiple homotypic K11 chains on the same substrate signal proteasomal degradation is unclear, but recently Lu et al. (2015) demonstrated that multiple short homotypic K48 chains on the cell-cycle substrate Securin are more efficient than homotypic K11 chains in stimulating proteasome degradation. "
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    ABSTRACT: Proteasome-mediated degradation occurs with proteins principally modified with lysine-48 polyubiquitin chains. Whether the proteasome also can bind atypical ubiquitin chains, including those linked by lysine-11, has not been well established. This is critically important, as lysine-11 polyubiquitination has been implicated in both proteasome-mediated degradation and non-degradative outcomes. Here we demonstrate that pure homotypic lysine-11-linked chains do not bind strongly to the mammalian proteasome. By contrast, heterotypic polyubiquitin chains, containing lysine-11 and lysine-48 linkages, not only bind to the proteasome but also stimulate the proteasomal degradation of the cell-cycle regulator cyclin B1. Thus, while heterotypic lysine-11-linked chains facilitate proteasomal degradation, homotypic lysine-11 linkages adopt conformations that prevent association with the proteasome. Our data demonstrate the capacity of the proteasome to bind ubiquitin chains of distinct topology, with implications for the recognition and diverse biological functions of mixed ubiquitin chains. Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.
    Cell Reports 07/2015; 12(4). DOI:10.1016/j.celrep.2015.06.061 · 8.36 Impact Factor
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    • "Chains that are linked through K48 are the principal signal for degradation by the proteasome [30] [31]. Recent studies, based on mass spectrometry have shown that homogeneous chains consisting of K29, K11, K27 and K6- linkages, heterogeneous chains with mixed lysine linkages, as well as multiple nearby monoubiquitination and, in cases of substrates up to 150 amino acids, even monoubiquitination can promote proteasomal degradation [32] [33]. Chain elongation of ubiquitinated substrates is mediated via another class of ubiquitin ligases, "
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    ABSTRACT: Posttranslational modification of proteins often controls various aspects of their cellular function. Indeed, over the past decade or so, it has been discovered that posttranslational modification of lysine residues plays a major role in regulating translesion DNA synthesis (TLS) and perhaps the most appreciated lysine modification is that of ubiquitination. Much of the recent interest in ubiquitination stems from the fact that proliferating cell nuclear antigen (PCNA) was previously shown to be specifically ubiquitinated at K164 and that such ubiquitination plays a key role in regulating TLS. In addition, TLS polymerases themselves are now known to be ubiquitinated. In the case of human polymerase η, ubiquitination at four lysine residues in its C-terminus appears to regulate its ability to interact with PCNA and modulate TLS. Within the past few years, advances in global proteomic research have revealed that many proteins involved in TLS are, in fact, subject to a previously underappreciated number of lysine modifications. In this review, we will summarize the known lysine modifications of several key proteins involved in TLS; PCNA and Y-family polymerases η, ι, κ and Rev1 and we will discuss the potential regulatory effects of such modification in controlling TLS in vivo. Copyright © 2015. Published by Elsevier B.V.
    DNA Repair 02/2015; 29. DOI:10.1016/j.dnarep.2015.02.011 · 3.11 Impact Factor
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    • "Ubiquitination generally occurs on primary amines (Lys, and, less frequently, a free N-terminus) as a consequence of nucleophillic attack on the E2~Ub linkage, resulting in stable isopeptide (or peptide) linkages with the C-terminus of ubiquitin. Monoubiquitination plays diverse roles in processes such as DNA repair, protein trafficking, and transcription [159], and recent findings have demonstrated that multi-monoubiquitination can also target proteins for proteasomal degradation [160] [161]. Alternatively, ubiquitin can be transferred to one of the seven Lys (K) or the N-terminal Met of ubiquitin molecules [162] (PDB 1UBQ) that are already substrate-linked, generating multi-or poly-ubiquitin chains. "
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    ABSTRACT: RING finger domain and RING finger-like ubiquitin ligases (E3s), such as U-box proteins, constitute the vast majority of known E3s. RING-type E3s function together with ubiquitin-conjugating enzymes (E2s) to mediate ubiquitination and are implicated in numerous cellular processes. In part because of their importance in human physiology and disease, these proteins and their cellular functions represent an intense area of study. Here we review recent advances in RING-type E3 recognition of substrates, their cellular regulation, and their varied architecture. Additionally, recent structural insights into RING-type E3 function, with a focus on important interactions with E2s and ubiquitin, are reviewed. This article is part of a Special Issue entitled:Ubiquitin-Proteasome System.
    Biochimica et Biophysica Acta 06/2013; 1843(1). DOI:10.1016/j.bbamcr.2013.05.026 · 4.66 Impact Factor
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