High-resolution biosensor based on localized surface plasmons

Institute of Photonics and Electronics, Academy of Sciences of the Czech Republic, Chaberská 57, Prague, Czech Republic.
Optics Express (Impact Factor: 3.49). 01/2012; 20(1):672-80. DOI: 10.1364/OE.20.000672
Source: PubMed


We report on a new biosensor with localized surface plasmons (LSP) based on an array of gold nanorods and the total internal reflection imaging in polarization contrast. The sensitivity of the new biosensor is characterized and a model detection of DNA hybridization is carried out. The results are compared with a reference experiment using a conventional high-resolution surface plasmon resonance (SPR) biosensor. We show that the LSP-based biosensor delivers the same performance as the SPR system while involving significantly lower surface densities of interacting molecules. We demonstrate a limit of detection of 100 pM and a surface density resolution of only 35 fg×mm-2 that corresponds to less than one DNA molecule per nanoparticle on average.

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Available from: Hana Sípová, Aug 18, 2014
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    • "Also the usage of single-and double-stranded DNA molecules as molecular ruler between two nanoparticles has been shown, concurrently being the first experiment where a DNA hybridization step has been demonstrated on a single particle level (Sönnichsen et al. 2005a). LSPR sensing with a limit of detection of 100 pM with an average density of less than one DNA molecule per involved gold nanostructures has been demonstrated (Piliarik et al. 2012). A parallel approach with an array consisting of hundreds of nanoparticles has shown a femtomolar detection limit for DNA hybridization events (Verdoold et al. 2011). "
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    ABSTRACT: The effect of DNA–DNA interaction on the localized surface plasmon resonance of single 80 nm gold nanoparticles is studied. Therefore, both the attachment of the capture DNA strands at the particle surface and the sequence-specific DNA bind-ing (hybridization) of analyte DNA to the immobilized capture DNA is subject of investigations. The influ-ence of substrate attachment chemistry, the packing density of DNA as controlled by an assisting layer of smaller molecules, and the distance as increased by a linker on the LSPR efficiency is investigated. The resulting changes in signal can be related to a higher hybridization efficiency of the analyte DNA to the immobilized capture DNA. The subsequent attach-ment of additional DNA strands to this system is studied, which allows for a multiple step detection of binding and an elucidation of the resulting resonance shifts. The detection limit was determined for the utilized DNA system by incubation with various concentration of analyte DNA. Although the method allows for a marker-free detection, we show that additional markers such as 20 nm gold particle labels increase the signal and thereby the sensitivity signif-icantly. The study of resonance shift for various DNA lengths revealed that the resonance shift per base is stronger for shorter DNA molecules (20 bases) as compared to longer ones (46 bases).
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    • "LSPRs are collective oscillations of conducting electrons in the nanoparticles, and they give rise to strongly enhanced and highly localized electromagnetic fields polarized perpendicularly to the metal surfaces. Compared to SPPs, LSPRs are much more localized and allow probing processes at the interface with spatial sensitivities on the nanometre scale [5] [6] [7]. LSPRs have been widely used in various sensing devices due to their high sensitivity to the refractive index of the surroundings [6] [7] [8] [9], as well as for enhancing Raman [10] [11], fluorescence [12] [13] [14] [15], IR [16] or second harmonic [17] signals. "
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    • "In case of infrared spectroscopy, an immunosensor characterized by a sensitivity as high as 3,022 nm/RIU and a LOD of 70 pg/mm2 has been demonstrated by Di Pippo et al. [64]. Recently, a high-resolution biosensor based on localized surface plasmon resonance (LSPR) excited on an array of gold nanorods have been proposed for detection of DNA hybridization [65]. A novel approach based on the imaging of surface plasmons in polarization contrast takes advantage of the change in the polarization of light coupled to LSP on a gold nanorod array. "
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