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Persistently bound Ku at DNA ends attenuates DNA end resection and homologous recombination

Division of Molecular Radiation Biology, Department of Radiation Oncology, The University of Texas Southwestern Medical Center, 2201 Inwood Rd, Dallas, TX 75390, USA.
DNA repair (Impact Factor: 3.36). 03/2012; 11(3):310-6. DOI: 10.1016/j.dnarep.2011.12.007
Source: PubMed

ABSTRACT DNA double strand breaks (DSBs) are repaired by non-homologous end joining (NHEJ) or homologous recombination (HR). The DNA cell cycle stage and resection of the DSB ends are two key mechanisms which are believed to push DSB repair to the HR pathway. Here, we show that the NHEJ factor Ku80 associates with DSBs in S phase, when HR is thought to be the preferred repair pathway, and its dynamics/kinetics at DSBs is similar to those observed for Ku80 in non-S phase in mammalian cells. A Ku homolog from Mycobacterium tuberculosis binds to and is retained at DSBs in S phase and was used as a tool to determine if blocking DNA ends affects end resection and HR in mammalian cells. A decrease in DNA end resection, as marked by IR-induced RPA, BrdU, and Rad51 focus formation, and HR are observed when Ku deficient rodent cells are complemented with Mt-Ku. Together, this data suggests that Ku70/80 binds to DSBs in all cell cycle stages and is likely actively displaced from DSB ends to free the DNA ends for DNA end resection and thus HR to occur.

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Available from: Jun Yang, Jul 30, 2015
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    • "Ku plays multiple roles during NHEJ. Initially, Ku not only binds and detects DSB but also protects the ends from nucleolytic degradation and tilts the choice of DSB repair towards NHEJ and away from HR [7] [36]. Perhaps the most extensive role for Ku is to recruit NHEJ factors to DSB sites. "
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