The effects of PHBV electrospun fibers with different diameters and orientations on growth behavior of bone-marrow-derived mesenchymal stem cells.
ABSTRACT Microenvironments in which cells live play an important role in the attachment, growth and interactions of cells. To mimic the natural structure of extracellular matrices, electrospinning was applied to fabricate biomaterials into ultrafine fibers. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), a biocompatible and biodegradable polyester, has been shown to be an excellent biomaterial candidate for tissue engineering. In this study, five types of PHBV fibrous scaffolds with different diameters and orientations were obtained by changing solvents, concentration of electrospun solution and collector. Three kinds of scaffolds with good continuity and suitable mechanical properties, selected according to the morphology and mechanical properties of the scaffolds, were used for studying the influence of fiber diameter and orientation on growth behavior of bone-marrow-derived mesenchymal stem cells (MSCs). The results indicated that the random-oriented nanofibrous scaffold is most favorable for cell growth compared to other scaffolds, while the microfibrous scaffold resulted in the lowest viability of MSCs. The orientation of nanofibers showed a distinct effect on cell morphology by guiding cell skeleton extension. Both the random-oriented and aligned PHBV nanofibrous scaffolds showed to be good candidates for applications in tissue engineering.
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ABSTRACT: The development of bioinspired or biomimetic materials is essential and has formed one of the most important paradigms in today's tissue engineering research. This paper reports a novel biomimetic nanocomposite nanofibers of hydroxyapatite/chitosan (HAp/CTS) prepared by combining an in situ co-precipitation synthesis approach with an electrospinning process. A model HAp/CTS nanocomposite with the HAp mass ratio of 30 wt% was synthesized through the co-precipitation method so as to attain homogenous dispersion of the spindle-shaped HAp nanoparticles (ca. 100 x 30 nm) within the chitosan matrix. By using a small amount (10 wt%) of ultrahigh molecular weight poly(ethylene oxide) (UHMWPEO) as a fiber-forming facilitating additive, continuous HAp/CTS nanofibers with a diameters of 214+/-25 nm had been produced successfully and the HAp nanoparticles with some aggregations were incorporated into the electrospun nanofibers. Further SAED and XRD analysis confirmed that the crystalline nature of HAp remains and had survived the acetic acid-dominant solvent system. Biological in vitro cell culture with human fetal osteoblast (hFOB) cells for up to 15 days demonstrated that the incorporation of HAp nanoparticles into chitosan nanofibrous scaffolds led to significant bone formation oriented outcomes compared to that of the pure electrospun CTS scaffolds. The electrospun nanocomposite nanofibers of HAp/CTS, with compositional and structural features close to the natural mineralized nanofibril counterparts, are of potential interest for bone tissue engineering applications.Biomaterials 12/2008; 29(32):4314-22. · 7.60 Impact Factor
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ABSTRACT: Electrospinning is regarded as a facile tool to generate biomaterials into a nanofibrous structure. Herein a nanofibrous web constituted of collagen and hydroxyapatite (HA) was produced from their co-precipitated nanocomposite solution by using the electrospinning method. The co-precipitated sol was freeze-dried and the dried product was dissolved in an organic solvent for the electrospinning. The electrospun web showed a well-developed nanofibrous structure with HA contents of up to 20 wt%. The internal structure of the collagen-20 wt%HA nanofiber revealed highly elongated apatite nanocrystallines precipitated within the collagen matrix. However, above the HA content of 30 wt% the nanofibrous structure could not be preserved due to the formation of beads. The MC3T3-E1 osteoblastic cells were shown to adhere and grow actively on the collagen-HA nanofibrous web. The alkaline phosphatase (ALP) activity expressed by the cells on the collagen-20 wt%HA nanofiber was lower at day 7, but was higher at day 14 than that on the pure collagen nanofiber. Based on the study, the newly-developed collagen-HA nanofiber may be useful as a cell supporting substrate in bone regeneration area.Journal of Materials Science Materials in Medicine 09/2008; 19(8):2925-32. · 2.14 Impact Factor
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ABSTRACT: Electrospun natural polymer membranes were fabricated from collagen or gelatin coated with a bioactive recombinant fragment of perlecan, a natural heparan sulfate proteoglycan. The electrospinning process allowed the facile processing of a three-dimensional, porous fibril (2-6 microm in diameter) matrix suitable for tissue engineering. Laser scanning confocal microscopy revealed that osteoblast-like MG63 cells infiltrated the depth of the electrospun membrane evenly without visible apoptosis. Tissue engineering scaffolds ideally mimic the extracellular matrix; therefore, the electrospun membrane must contain both structural and functional matrix features. Fibers were coated, after processing, with perlecan domain I (PlnDI) to improve binding of basic fibroblast growth factor (FGF-2), which binds to native heparan sulfate chains on PlnDI. PlnDI-coated electrospun collagen fibers were ten times more effective than heparin-BSA collagen fibers at binding FGF-2. Because FGF-2 modulates cell growth, differentiation, migration and survival, the ability to effectively bind FGF-2 to an electrospun matrix is a key improvement in creating a successful tissue engineering scaffold.Biomacromolecules 05/2007; 8(4):1116-23. · 5.37 Impact Factor