The promise of induced pluripotent stem cells in research and therapy.

Stem Cell Transplantation Program, Division of Pediatric Hematology/Oncology, Manton Center for Orphan Disease Research, Howard Hughes Medical Institute, Children's Hospital Boston and Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA.
Nature (Impact Factor: 42.35). 01/2012; 481(7381):295-305. DOI: 10.1038/nature10761
Source: PubMed

ABSTRACT The field of stem-cell biology has been catapulted forward by the startling development of reprogramming technology. The ability to restore pluripotency to somatic cells through the ectopic co-expression of reprogramming factors has created powerful new opportunities for modelling human diseases and offers hope for personalized regenerative cell therapies. While the field is racing ahead, some researchers are pausing to evaluate whether induced pluripotent stem cells are indeed the true equivalents of embryonic stem cells and whether subtle differences between these types of cell might affect their research applications and therapeutic potential.

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    ABSTRACT: Directed differentiation of human pluripotent stem cells (hPSCs) into mature cells, tissues and organs holds major promise for the development of novel approaches in regenerative medicine, and provides a unique tool for disease modeling and drug discovery. Sometimes underappreciated is the fact that directed differentiation of hPSCs also provides a unique model for human development, with a number of important advantages over model organisms. Here, I discuss the importance of using human stem cell models for understanding human lung development and disease. © 2015. Published by The Company of Biologists Ltd.
    Development 01/2015; 142(1):13-6. · 6.27 Impact Factor
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    American Journal of Biomedical Research. 01/2013; 1(2):35-42.
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    ABSTRACT: Introduction The differentiated cell lineages from human em-bryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) have shown their potential in regenerative medicine. However, the functional and transcriptional microRNA (miRNA) expression pattern during endothelial differentiation has yet to be characterized. Methods In this study, hESCs and hiPSCs were differentiated into endothelial cells (ECs). Then the endothelial-related gene profiling and miRNA profiling of hiPSCs, hESCs, hiPSCs derived endothelial cells (hiPSC-ECs), hESC derived endo-thelial cells (hESC-ECs) and human umbilical vein endothe-lial cells (HUVECs) were compared using RT-PCR Array. The data was analyzed using the data analysis system on QIAGEN's website. Results Our analysis demonstrated that the endothelial differ-entiation was triggered after EB formation and the EC-associated genes were up-regulated swiftly in both hESC-EBs and hiPSC-EBs; hiPSC-ECs and hESC-ECs had the sim-ilar EC-associated gene expression patterns. Moreover, we re-port here the first miRNA profiling study of hiPSC-ECs. An-alyzing 376 unique miRNAs, we have identified several inter-esting miRNAs, including miR-20a, miR-20b, miR-222, miR-210, which have been previously reported to be involved in endothelial differentiation and show surprising expression pat-terns across our samples. We also identified novel miRNAs, such as miR-125a-5p, miR-149, miR-296-5p, miR-100, miR-27b, miR-181a and miR-137, which were up-regulated in both hiPSC-ECs and hESC-ECs during endothelial differentiation. Conclusion hiPSC-ECs and hESC-ECs exhibited a high de-gree of similarity with HUVECs in EC-associated genes ex-pression. And the miRNA profiling analysis revealed signifi-cant differences between hiPSCs and hESCs, but a high de-gree of similarity between hiPSC-ECs and hESC-ECs.
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