Article

The TAGteam motif facilitates binding of 21 sequence-specific transcription factors in the Drosophila embryo.

Department of Statistics, Oxford University, Oxford OX1 3TG, United Kingdom;
Genome Research (impact factor: 13.61). 02/2012; 22(4):656-65. DOI:10.1101/gr.130682.111 pp.656-65
Source: PubMed

ABSTRACT Highly overlapping patterns of genome-wide binding of many distinct transcription factors have been observed in worms, insects, and mammals, but the origins and consequences of this overlapping binding remain unclear. While analyzing chromatin immunoprecipitation data sets from 21 sequence-specific transcription factors active in the Drosophila embryo, we found that binding of all factors exhibits a dose-dependent relationship with "TAGteam" sequence motifs bound by the zinc finger protein Vielfaltig, also known as Zelda, a recently discovered activator of the zygotic genome. TAGteam motifs are present and well conserved in highly bound regions, and are associated with transcription factor binding even in the absence of canonical recognition motifs for these factors. Furthermore, levels of binding in promoters and enhancers of zygotically transcribed genes are correlated with RNA polymerase II occupancy and gene expression levels. Our results suggest that Vielfaltig acts as a master regulator of early development by facilitating the genome-wide establishment of overlapping patterns of binding of diverse transcription factors that drive global gene expression.

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    [show abstract] [hide abstract]
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Keywords

21 sequence-specific transcription factors active
 
canonical recognition motifs
 
chromatin immunoprecipitation data sets
 
discovered activator
 
distinct transcription factors
 
diverse transcription factors
 
dose-dependent relationship
 
drive global gene expression
 
Drosophila embryo
 
factors exhibits
 
genome-wide binding
 
master regulator
 
overlapping binding
 
overlapping patterns
 
RNA polymerase II occupancy
 
transcription factor binding
 
Vielfaltig acts
 
zinc finger protein Vielfaltig
 
zygotic genome
 
zygotically transcribed genes
 

Rahul Satija