RACK1 Suppresses Gastric Tumorigenesis by Stabilizing the β-Catenin Destruction Complex

Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
Gastroenterology (Impact Factor: 16.72). 01/2012; 142(4):812-823.e15. DOI: 10.1053/j.gastro.2011.12.046
Source: PubMed


Dysregulation of Wnt signaling has been involved in gastric tumorigenesis by mechanisms that are not fully understood. The receptor for activated protein kinase C (RACK1, GNB2L1) is involved in development of different tumor types, but its expression and function have not been investigated in gastric tumors.
We analyzed expression of RACK1 in gastric tumor samples and their matched normal tissues from 116 patients using immunohistochemistry. Effects of knockdown with small interfering RNAs or overexpression of RACK1 in gastric cancer cell lines were evaluated in cell growth and tumor xenograft. RACK1 signaling pathways were investigated in cells and zebrafish embryos using immunoblot, immunoprecipitation, microinjection, and in situ hybridization assays.
Expression of RACK1 was reduced in gastric tumor samples and correlated with depth of tumor infiltration and poor differentiation. Knockdown of RACK1 in gastric cancer cells accelerated their anchorage-independent proliferation in soft agar, whereas overexpression of RACK1 reduced their tumorigenicity in nude mice. RACK1 formed a complex with glycogen synthase kinase Gsk3β and Axin to promote the interaction between Gsk3β and β-catenin and thereby stabilized the β-catenin destruction complex. On stimulation of Wnt3a, RACK1 repressed Wnt signaling by inhibiting recruitment of Axin by Dishevelled 2 (Dvl2). Moreover, there was an inverse correlation between expression of RACK1 and localization of β-catenin to the cytoplasm/nucleus in human gastric tumor samples.
RACK1 negatively regulates Wnt signaling pathway by stabilizing the β-catenin destruction complex and act as a tumor suppressor in gastric cancer cells.

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    • "Several Axin-binding proteins have been identified that modulate the phosphorylation of β-catenin in the Axin–GSK3 complex (Haraguchi et al., 2008; N.G. Kim et al., 2009; Kashikar et al., 2011; Deng et al., 2012), suggesting that much remains to be learned about how signaling pathways are integrated at this locus. Others have reviewed the details of the β-catenin destruction complex (MacDonald et al., 2009; Valvezan & Klein, 2012; Willert & Nusse, 2012), but there are several important points that are often overlooked by investigators new to the field. "
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