Molecular differentiation of Chenopodium album complex and some related species using ISSR profiles and ITS sequences
Conservation Biology and Molecular Taxonomy, CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow 226 001, India. Gene
(Impact Factor: 2.14).
03/2012; 495(1):29-35. DOI: 10.1016/j.gene.2011.12.031
The present study was undertaken to understand the genetic differentiation and relationships in various components of C. album complex, C. giganteum and some related species using inter simple sequence repeats (ISSR) profiles and internal transcribed spacer (ITS) sequences. The relationships based on UPGMA dendrograms have shown the heterogenous nature of C. album complex. The 2x taxa while showing close relation among themselves are sharply segregated from 4x and 6x taxa belonging to C. album and C. giganteum. Among the three cytotypes from North Indian plains the 4x shows greater similarity to 6x than to 2x which is corroborated by the karyotypic studies. Furthermore, the 6x C. album and C. giganteum accessions of American and European origin are clearly segregated from those of Indian origin which may show their separate origin. Other related species show relationships according to their taxonomic position. The present study based on ISSR profiles and ITS sequences has therefore been very useful in explaining the relationships between various components of C. album complex and related species. However, more work needs to be done using different CpDNA loci to define correct species boundary of the taxa under C. album complex from Himalayas and North Indian Plains.
Available from: Fabio Mendonça Diniz
- "The assessment of the genetic diversity present within Melipona subnitida populations is therefore a prerequisite for the establishment of efficient management and conservation practices. Molecular markers have proven to be decisive in elucidating diversity and differences at the DNA level in microorganisms, plants and animals (Panwar et al. 2010; Sebastien et al. 2012; Rana et al. 2012; Bonatti et al. 2014). Among several markers, Inter Simple Sequence Repeats (ISSR) have been used due to their low cost and high level of polymorphism, and as an alternative to overcome reproducibility problems commonly found in other PCR-based markers (Abbot 2001; Lima-Brito et al. 2011). "
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ABSTRACT: For a snapshot assessment of the genetic diversity present within Melipona subnitida, an endemic stingless bee distributed in the semi-arid region of northeastern Brazil, populations separated by over 1,000 km distance were analyzed by ISSR genotyping. This is a prerequisite for the establishment of efficient management and conservation practices. From 21 ISSR primers tested, only nine revealed consistent and polymorphic bands (loci). PCR reactions resulted in 165 loci, of which 92 were polymorphic (57.5%). Both Phi(ST) (ARLEQUIN) and theta(B) (HICKORY) presented high values of similar magnitude (0.34, p<0.0001 and 0.33, p<0.0001, respectively), showing that these two groups were highly structured. The dendrogram obtained by the cluster analysis and the scatter-plot of the PCoA corroborate with the data presented by the AMOVA and theta(B) tests. Clear evidence of subdivision among sampling sites was also observed by the Bayesian grouping model analysis (STRUCTURE) of the ISSR data. It is clear from this study that conservation strategies should take into account the heterogeneity of these two separate populations, and address actions towards their sustainability by integrating our findings with ecological tools.
Journal of Hymenoptera Research 06/2014; 38(1):1-9. DOI:10.3897/JHR.38.7302 · 0.90 Impact Factor
Available from: Duane Fernandes Lima
- "Single-locus molecular markers have been successfully used as a biosystematic tool, in order to define interspecific limits within species complexes (Crawford and Mort 2004). Among these, the ISSR (inter simple sequence repeat, Zietkiewicz et al. 1994) is very useful for detecting genetic polymorphisms at species level (Gaiero et al. 2011; Amirmoradi et al. 2012; Rana et al. 2012), since it produces a large number of polymorphic fragments that can be interpreted as multiloci genetic profiles (Zietkiewicz et al. 1994). Myrtaceae is one of these plant families where identification problems are very common (Biffin et al. 2010). "
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ABSTRACT: Myrtaceae is a family with high taxonomic complexity, especially the very rich genera such as Myrcia. For this genus, there is not a complete and recent taxonomic revision, and it has some species complexes that are poorly delimited. One of these complexes includes M. laruotteana, M. selloi, M. lajeana and M. tomentosa. In order to better understand the species boundaries in this complex, we analyzed the genetic variability and structure of multiple populations of these four species using inter simple sequence repeat markers, checking if there is any relationship between genetic, morphological and geographical patterns. The amount of genetic diversity found was similar (H
E = 0.215) compared to species with similar biological characteristics. No groupings that were previously tested (morphological, geographical and the ones used in the traditional taxonomy) showed significant genetic structure. The groups suggested by Bayesian analysis showed a higher genetic structure through analyses of molecular variance: 12 % of the variation between populations within groups and 27 % of the variation between groups. The Bayesian analysis and the neighbor-joining dendrogram showed two major groups, the first with all populations of M. tomentosa, and the second containing populations from the other species. According to the unified species concept, there is enough evidence for the recognition of M. tomentosa,
M. laruotteana and M. selloi, but not for M. lajeana. These data will be used as the basis of a new taxonomic classification of the species in the M. laruotteana complex.
Plant Systematics and Evolution 06/2014; 301(1). DOI:10.1007/s00606-014-1078-9 · 1.42 Impact Factor
Available from: Luc Legal
- "In parallel, at the same taxonomic level, many other plant studies are based on gene sequencing data from nuclear or chloroplast DNA (Naciri and Gaudeul, 2007; Ruiz-Sanchez and Sosa, 2010). Finally, a number of papers include both methods, ISSR and gene sequencing, to characterize the relationships within species complexes yielding a more reliable data set (Li et al., 2008a; Meimberg et al., 2006; Rana et al., 2012). "
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ABSTRACT: The taxonomy and systematics of Mexican Lupinus are lacking in resolution, because the taxa are distinguished using a few minor and inconsistent morphological characters. The use of molecular markers can contribute to re-solving such issues. In this study, we focused on two varieties of the Lupinus montanus complex (Fabaceae) in Mexico, L. montanus subsp. montanus var. montanus and L. montanus subsp. montanus var. nelsonii, and aimed to determine the most suitable genetic markers for clarifying taxon delimitation based on morphology. We com-pared hypervariable Inter Simple Sequence Repeats (ISSR) to gene sequences (External Transcribed Spacer (ETS) and Conserved Orthologous Sets (COS)). Distance analysis (ISSR) and maximum likelihood (ETS + COS) generated congruent results but with much more variability and resolution for ISSR. These data confirm the potential of ISSR for working at low taxonom-ic level and their reliability compared to that of gene sequences.
South African Journal of Botany 08/2013; 89:106-110. DOI:10.1016/j.sajb.2013.06.021 · 0.98 Impact Factor
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