Article

Modern electron microscopy methods for C. elegans.

Center for C. elegans Anatomy, Albert Einstein College of Medicine, Bronx, New York, USA.
Methods in cell biology (impact factor: 2.05). 01/2012; 107:93-149. DOI:10.1016/B978-0-12-394620-1.00004-7
Source: PubMed

ABSTRACT From its inception as a model organism 40 years ago, Caenorhabditis elegans was chosen in part for its suitability for study in serial thin sections by electron microscopy. Recent improvements in electron microscopy technology are making this pursuit more reliable and more powerful. In this chapter, we highlight new methods in specimen preparation, imaging, and data analysis. Accurate three-dimensional information can now be obtained for the whole animal at all stages, down to the level of individual organelles and the cytoskeleton.

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Keywords

Accurate three-dimensional information
 
cytoskeleton
 
data analysis
 
electron microscopy technology
 
model organism 40 years
 
new methods
 
serial thin sections
 
suitability
 
whole animal
 

David H Hall