Silanimonas mangrovi sp. nov., a novel bacterium of the family Xanthomonadaceae isolated from mangrove sediment and emended description of the genus Silanimonas.

Abstract

A novel Gram-negative, rod shaped, motile bacterium, designated strain AK13T, was isolated from a sediment sample collected from mangrove of Namkhana, Sunderbans, West Bengal, India. Strain AK13T was positive for oxidase, DNase and lipase activities and negative for catalase, gelatinase, ornithine decarboxylase, lysine decarboxylase, nitrate reductase, aesculinase and urease activities. The fatty acids were dominated by iso-C11 : 0, iso-C11 : 0 3OH, iso-C15 : 0, iso-C16 : 0, iso-C17 : 1 ω9c and summed in feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2OH). Strain AK13T contained Q-8 as the major respiratory quinone and diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylserine, two unidentified aminolipids, one unidentified glycolipid and one unidentified lipid as polar lipids. The DNA G + C content of the strain AK13T was 55.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequence of strain AK13T indicated Silanimonas lenta of the family Xanthomonadaceae (phylum Proteobacteria) as the closest related species with sequence similarity of 95.2%. Other members of the family showed sequence similarities <94.4%. Based on the phenotypic characteristics and on phylogenetic inference, strain AK13T is proposed as a novel species of the genus Silanimonas as Silanimonas mangrovi sp. nov. and the type strain is AK13T (= MTCC 11082T = DSM 24914T).