Article

Knockdown of Ebola virus VP24 impairs viral nucleocapsid assembly and prevents virus replication.

Filovirus Laboratory, INSERM U758, Human Virology Department, Université de Lyon, Ecole Normale Supérieure de Lyon, Lyon, France.
The Journal of Infectious Diseases (impact factor: 6.41). 11/2011; 204 Suppl 3:S892-6. DOI:10.1093/infdis/jir311 pp.S892-6
Source: PubMed

ABSTRACT The structural protein VP24 of Ebola virus (EBOV) is a determinant of virulence in rodent models and possesses an interferon antagonist function. In this study, we investigate the role of VP24 in EBOV replication using RNA interference by small interfering RNA to knock down the expression of this protein in virus-infected cells. We reveal that VP24 is required for assembly of viral nucleocapsids and that silencing of VP24 expression prevents the release of EBOV.

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Keywords

determinant
 
Ebola virus
 
interferon antagonist function
 
possesses
 
RNA interference
 
rodent models
 
structural protein VP24
 
viral nucleocapsids
 
virulence
 
virus-infected cells