Epidemiological and molecular analysis of a waterborne outbreak of norovirus GII.4.

Guangdong Provincial Centre for Disease Control and Prevention, Guangdong, China.
Epidemiology and Infection (Impact Factor: 2.49). 03/2012; DOI: 10.1017/S0950268812000374
Source: PubMed

ABSTRACT SUMMARYContaminated water is one of the main sources of norovirus (NoV) gastroenteritis outbreaks globally. Waterborne NoV outbreaks are infrequently attributed to GII.4 NoV. In September 2009, a NoV outbreak affected a small school in Guangdong Province, China. Epidemiological investigations indicated that household use water, supplied by a well, was the probable source (relative risk 1·9). NoV nucleic acid material in concentrated well-water samples was detected using real-time RT-PCR. Nucleotide sequences of NoV extracted from diarrhoea and well-water specimens were identical and had the greatest sequence identity to corresponding sequences from the epidemic strain GII.4-2006b. Our report documents the first laboratory-confirmed waterborne outbreak caused by GII.4 NoV genotype in China. Our investigations indicate that well water, intended exclusively for household use but not for consumption, caused this outbreak. The results of this report serve as a reminder that private well water intended for household use should be tested for NoV.


Available from: John D Klena, Jan 16, 2014
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    PLoS ONE 09/2014; 9(9):e105053. DOI:10.1371/journal.pone.0105053 · 3.53 Impact Factor
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    ABSTRACT: To provide a rapid and sensitive method for detecting NoV GI and NoV GII in water and to evaluate the use of the murine norovirus (MNV-1) as a process control. The method is based on viral concentration by filtration on electropositive filters and direct lysis of adsorbed viruses from filters before RNA extraction and RT-qPCR amplification. A one-step multiplex RT-qPCR assay was developed for the simultaneous detection of NoV GI, NoV GII and MNV-1. Then, water samples were artificially contaminated to determine mean virus recoveries and method sensitivity. The method showed a higher sensitivity for detecting NoV GII (10(3) genome copies /0.5 L) than for NoV GI (10(4) genome copies /0.5 L) in presence of MNV-1 regardless of the type of water. The data also showed that MNV-1 is a robust option as process control. The method described provides a valuable tool for the monitoring of potential public health risks associated with NoV contamination in drinkable water. Given the increasing evidence for NoV involvement in food outbreaks, the one step multiplex RT-qPCR assay we used in this study would be a very useful tool to investigate NoV contamination in other food products. This article is protected by copyright. All rights reserved.
    Journal of Applied Microbiology 09/2013; 116(1). DOI:10.1111/jam.12345 · 2.39 Impact Factor
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