Conference Paper

Automated Analysis of siRNA Screens of Virus Infected Cells Based on Immunofluorescence Microscopy.

DOI: 10.1007/978-3-540-78640-5_91 Conference: Bildverarbeitung für die Medizin 2008, Algorithmen, Systeme, Anwendungen, Proceedings des Workshops vom 6. bis 8. April 2008 in Berlin
Source: DBLP

ABSTRACT We present an image analysis approach as part of a high- throughput microscopy screening system based on cell arrays for the identification of genes involved in Hepatitis C and Dengue virus replica- tion. Our approach comprises: cell nucleus segmentation, quantification of virus replication level in cells, localization of regions with transfected cells, cell classification by infection status, and quality assessment of an experiment. The approach is fully automatic and has been successfully applied to a large number of cell array images from screening experi- ments. The experimental results show a good agreement with the ex- pected behavior of positive as well as negative controls and encourage the application to screens from further high-throughput experiments.

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