Article

Enrichment of Artemia nauplii with Lactobacillus sporogenes for Enhancing the Survival, Growth and Levels of Biochemical Constituents in the Post-Larvae of the Freshwater Prawn Macrobrachium rosenbergii

Turkish Journal of Fisheries and Aquatic Sciences (Impact Factor: 0.38). 04/2012; DOI: 10.4194/1303-2712-v12_1_04

ABSTRACT In this study, the live feed, Artemia franciscana naupli was enriched with probiotic bacterium, Lactobacillus sporogenes and fed to the freshwater prawn, Macrobrachium rosenbergii post larvae (PL) for attaining good survival, growth and contents of biochemical constituents. Artemia was enriched with different concentrations of L. sporogenes (15x107, 30x107, 45x107 and 60x107 cfu cells) and fed to M. rosenbergii PL (1.5 cm length and 0.03 g weight) for a period of 45 days. The PL fed with 60x107 cfu cells of L. sporogenes enriched Artemia produced significantly (P<0.05) higher survival and growth when compared to that of other concentrations of L. sporogenes, and the control fed with un-enriched Artemia. The biomass increase, total weight gain, specific growth rate, condition factor and mean conversion ratio were found to be higher in PL fed with L. sporogenes enriched Artemia when compared with control. The enhanced growth performance of PL fed with L. sporogenes was further confirmed by the lower food conversion ratio recorded. The levels of biochemical constituents, such as total protein, amino acids, carbohydrate and lipid contents were found to significantly higher (P<0.05) in PL fed on L. sporogenes enriched Artemia particularly at 60x107 cfu cells when compared to that of control. Significantly (P<0.05) higher ash and lower moisture contents were also observed in PL fed with 60x107 cfu cells of L. sporogenes enriched Artemia. Therefore, it is evident that 60x107 cfu cells of L. sporogenes can be considered as suitable concentration for attaining good survival and growth of M. rosenbergeii PL.

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Available from: Dr. Chandrasekar Seenivasan, Aug 09, 2015
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    • "The presence of above said six isolates were identified as bacterial colonies and confirmed through routine biochemical tests (Tables 3-6). In M. rosenbergii PL the establishment of the probiotic bacteria, L. sporogenes and L. acidophilus have been reported (Venkat et al. 2004; Seenivasan et al. 2012a). The replacement of Vibrio spp., by Bacillus S11 in the shrimp, P. monodon increases survival rate (Rengpipat et al. 1998). "
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    ABSTRACT: A probiotic yeast, Saccharomyces cerevisiae was incorporated in basal diet prepared with fish meal, soybean meal, groundnut oil cake, corn flour, tapioca flour, egg albumin, cod liver oil and vitamin B-complex, at four different concentrations (10g, 20g, 30g and 40g kg -1) and fed to Macrobrachium rosenbergii post larvae (PL) for 90 days. The effect of this probiotic incorporation on the growth and survival performances, concentration of protein, amino acid, carbohydrate and lipid, and energy utilization was found to be significantly (P<0.05) higher at 40g kg -1 followed by 30g, 20g and 10g kg -1 . 40g kg -1 S. cerevisiae incorporation was found to be established the highest rate of colony formation, 234x10 -4 cfu (colony formation units). Actually, presence of Bacillus spp., Bacillus cereus, Pseudomonas spp., Escherichia coli, Streptococcus spp., and Klebsiella pneumoniae were deducted in water medium and the PL gut of control group. There is a general belief that Pseudomonas spp. and K. pneumonia are pathogenic to prawns. The establishment of S. cerevisiae colony in the gut of experimental PL has eradicated these pathogenic bacteria. Therefore, it is suggested that establishment of S. cerevisiae colony has led to better growth, survival and biochemical constituents in M. rosenbergii PL. Thus, S. cerevisiae could be taken as a useful probiotic in M. rosenbergii culture.
    International Journal of Applied Biology and Pharmaceutical Technology 07/2013; 4(3):39-47. · 0.99 Impact Factor
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    • "All necessary confirmation biochemical tests were performed and the results were presented (Tables 5–7; Fig. 1). In agreement with the present findings, Seenivasan et al. (2012a–d) and Zhang et al. (2009) stated that the dietary probiotics L. sporogenes, B. subtilis and S. cerevisiae and probiotic B12 had significantly increased the total bacterial counts in intestines of the M. rosenbergii and Fenneropenaeus chinensis respectively. Robertson et al. (2000) found that a great deal of probiotics can be detected in the shrimp intestine even after 3 days of withdrawal of the probiotic diet. "
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    ABSTRACT: This experiment was carried out to evaluate the effects of the probiotic Bacillus subtilis on survival, growth, biochemical constituents and energy utilization in Macrobrachium rosenbergii PL. Experimental diets were the same in all, except for the variation in probiotic levels. The probiotic B. subtilis was used at 0% (diet 1), 1% (diet 2), 2.0% (diet 3), 3.0% (diet 4) and 4.0% (diet 5) inclusion in the experimental diets. These diets were fed to M. rosenbergii PL for a period of 90 days. The growth parameters, such as survival, weight gain, specific growth rate, feed conversion efficiency and protein efficiency rate were significantly (P < 0.05) higher in 3% B. subtilis incorporated diet fed PL followed by other experimental groups than the control. Similarly the biochemical constituents of the protein, amino acid, carbohydrate, lipid and ash content were significantly (P <0.05) higher in 3% B. subtilis incorporated diet fed PL. The energy utilization parameters, such as feeding rate, absorption rate, conversion rate and metabolic rate were significantly (P <0.05) higher in 3% B. subtilis incorporated diet fed PL. From the results of this experiment, we conclude that B. subtilis can be used as a probiotic in M. rosenbergii culture, to enhance the survival, growth and improved energy utilization performance.
    01/2013; 38(3). DOI:10.1016/j.ejar.2012.12.009
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    • "These differences were found to be statistically significant (P<0.05). Similar results reported by Seenivasan et al. [4] tested different concentrations of L. sporogenes bioencapsulated Artemia fed M. rosenbergii PL had significantly enhanced the final length and weight than the control diet fed PL group. It has been reported by Deeseenthum et al. [10] showed that feeding M. rosenbergii PL with commercial diets containing 10 7 cfu ml -1 of Bacillus spp KKU02 and Bacillus spp KKU03, enhanced growth performance than control diet fed prawn PL. "
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    ABSTRACT: A 90-day feeding experiment was studied to determine the influence of the bacterial combination Lactobacillus sporogenes and Bacillus subtilis (LS+BS) on survival, growth, biochemical constituents and energy utilization performance of the freshwater prawn Macrobrachium rosenbergii post larvae (PL). Experimental diets were the same, except for the variation in probiotic levels. The probiotics LS+BS (4:3) were used at 0%, 1%, 2%, 3% and 4% inclusion rates in the experimental diets. After the feeding trail, the growth parameters of the PLs, such as survival, weight gain, specific growth rate, feed conversion efficiency and protein efficiency rate were significantly (P<0.05) higher in 4% LS+BS incorporated diet as compared with control. The food conversion ratio was significantly (P<0.05) lower in 4% LS+BS incorporated diet. The biochemical composition of the total protein, amino acid, carbohydrate and lipid ash content were significantly (P<0.05) higher in 4% LS+BS incorporated diet. However, insignificant difference was recorded in moisture content between control and experimental groups. The energy utilization parameters, such as feeding rate, absorption rate, conversion rate and metabolic rate were significantly (P<0.05) higher in 4% LS+BS incorporated diet fed PL. These results revealed the benefits of the incorporation of the probiotic (LS+BS) in aqua feed for M. rosenbergii PL.
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