Article

Enrichment of Artemia nauplii with Lactobacillus sporogenes for Enhancing the Survival, Growth and Levels of Biochemical Constituents in the Post-Larvae of the Freshwater Prawn Macrobrachium rosenbergii

Turkish Journal of Fisheries and Aquatic Sciences (Impact Factor: 0.59). 04/2012;

ABSTRACT In this study, the live feed, Artemia franciscana naupli was enriched with probiotic bacterium, Lactobacillus sporogenes and fed to the freshwater prawn, Macrobrachium rosenbergii post larvae (PL) for attaining good survival, growth and contents of biochemical constituents. Artemia was enriched with different concentrations of L. sporogenes (15x107, 30x107, 45x107 and 60x107 cfu cells) and fed to M. rosenbergii PL (1.5 cm length and 0.03 g weight) for a period of 45 days. The PL fed with 60x107 cfu cells of L. sporogenes enriched Artemia produced significantly (P<0.05) higher survival and growth when compared to that of other concentrations of L. sporogenes, and the control fed with un-enriched Artemia. The biomass increase, total weight gain, specific growth rate, condition factor and mean conversion ratio were found to be higher in PL fed with L. sporogenes enriched Artemia when compared with control. The enhanced growth performance of PL fed with L. sporogenes was further confirmed by the lower food conversion ratio recorded. The levels of biochemical constituents, such as total protein, amino acids, carbohydrate and lipid contents were found to significantly higher (P<0.05) in PL fed on L. sporogenes enriched Artemia particularly at 60x107 cfu cells when compared to that of control. Significantly (P<0.05) higher ash and lower moisture contents were also observed in PL fed with 60x107 cfu cells of L. sporogenes enriched Artemia. Therefore, it is evident that 60x107 cfu cells of L. sporogenes can be considered as suitable concentration for attaining good survival and growth of M. rosenbergeii PL.

0 0
 · 
0 Bookmarks
 · 
111 Views
  • Source
    [show abstract] [hide abstract]
    ABSTRACT: A 90-day feeding experiment was studied to determine the influence of the bacterial combination Lactobacillus sporogenes and Bacillus subtilis (LS+BS) on survival, growth, biochemical constituents and energy utilization performance of the freshwater prawn Macrobrachium rosenbergii post larvae (PL). Experimental diets were the same, except for the variation in probiotic levels. The probiotics LS+BS (4:3) were used at 0%, 1%, 2%, 3% and 4% inclusion rates in the experimental diets. After the feeding trail, the growth parameters of the PLs, such as survival, weight gain, specific growth rate, feed conversion efficiency and protein efficiency rate were significantly (P<0.05) higher in 4% LS+BS incorporated diet as compared with control. The food conversion ratio was significantly (P<0.05) lower in 4% LS+BS incorporated diet. The biochemical composition of the total protein, amino acid, carbohydrate and lipid ash content were significantly (P<0.05) higher in 4% LS+BS incorporated diet. However, insignificant difference was recorded in moisture content between control and experimental groups. The energy utilization parameters, such as feeding rate, absorption rate, conversion rate and metabolic rate were significantly (P<0.05) higher in 4% LS+BS incorporated diet fed PL. These results revealed the benefits of the incorporation of the probiotic (LS+BS) in aqua feed for M. rosenbergii PL.
    Journal of Ecobiotechnology. 06/2012;

Full-text

View
49 Downloads
Available from
Jun 28, 2012