Nucleotide specific changes in the hypervariable region of 16S rDNA gene as possible marker to differentiate the tick genera.

The Indian journal of animal sciences (Impact Factor: 0.16). 01/2011; 18(12):1204-1207.

ABSTRACT Hyper variable segment of mitochondrial 16S rDNA from different stages of laboratory reared, disease free and
acaricide susceptible Hyalomma anatolicum and Rhipicephalus (Boophilus) microplus were partially amplified, sequenced
and analyzed with the aid of the GenBank database. Thirty conserved genus specific nucleotide change were observed
in Hyalommid and Boophilid ticks. These conserved sequences were sufficient to identify embryonic stages of the
ticks. These conserved sequences at the genus level could act as biomarker for identification of ticks during
epidemiological studies of tick borne diseases, transmitted by Hyalommid and Rhipicephalid ticks.

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Available from: Sachin Kumar, Sep 29, 2015
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    • "The genetic homogeneity amongst different generations of the reference line was established by obtaining consistent entomological data and by analyzing the sequences of 16s rRNA gene of the tick species (Accession nos. GU222462, GU323287, GU323288) (Kumar Rinesh et al., 2011). Both larvae and adults were maintained at 28 • C and 85 ± 5% RH and were used for determination of DC of targeted acaricides. "
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    ABSTRACT: Discriminating concentrations (DCs) of malathion, coumaphos, fenvalerate and fipronil were determined to monitor acaricide resistance in field conditions. The LC99 values with 95% confidence interval for malathion, coumaphos, fenvalerate and fipronil were 5126.8 (5011.5-5240.7), 131.0 (120.4-142.5), 2257.5 (2198.1-2318.4) and 6.2 (5.87-6.55), respectively. The narrow confidence intervals in LC50 and LC99 of adult immersion test (AIT) and larval packet test (LPT) affirming the homogeneity of IVRI-I line. Variation in LPT based LC50 and LC99 values of malathion (55.9ppm) and coumpahos (28.4ppm) compared to those obtained in AIT indicating that larvae were more susceptible to these chemicals. The DCs for malathion, coumaphos, fenvalerate and fipronil against adults were determined as 10253.6, 262.0, 4515.0 and 12.4ppm while against larvae the values were 111.8, 56.8, 4014.0 and 9.6ppm, respectively. The working efficiency of DCs was successfully tested in field tick isolates. Establishment of country specific DCs of commonly used insecticides for monitoring of resistance in field ticks is emphasized for establishing tick control strategies. Copyright © 2015 Elsevier GmbH. All rights reserved.
    Ticks and Tick-borne Diseases 03/2015; 6(3). DOI:10.1016/j.ttbdis.2015.03.003 · 2.72 Impact Factor
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    • "The homogeneity amongst different generations of IVRI-I line was established by uniform entomological parameters and by analyzing the sequences of 16s rDNA gene (Accession Nos. GU222462, GU323287, GU323288 (Kumar Rinesh et al. 2011). "
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    ABSTRACT: Field tick isolates of Rhipicephalus (Boophilus) microplus were collected from eleven districts located in the northern and eastern states of India to access the resistance status to "Amitraz". Adult immersion test was optimized using laboratory reared acaricide susceptible IVRI-I line and minimum effective concentration was determined as 487.7 ppm with 95 % confidence interval of 455.8-521.8. The discriminating concentration was determined as 975.4 ppm and was tested on female ticks collected by two stage stratified sampling from organized dairy farms and villages. Based on three variables, viz., mortality, egg masses and reproductive index, the resistance level was categorized. Resistance to amitraz was detected at level I in 3 isolates (RF = 1.56-5.0), at level II in 6 isolates (RF = 9.3-23.3) and at level III in 1 isolate (RF = 27.3) whereas one isolate was found susceptible. The highest resistance was found in the SKR isolate (RF = 27.3) and minimal resistance was detected in the N-24P isolate (RF = 1.56). These experimental data will help in designing tick control strategy which is suffering from acaricide failure and to overcome development of resistance in ticks.
    Experimental and Applied Acarology 03/2014; 63(3). DOI:10.1007/s10493-014-9789-8 · 1.62 Impact Factor
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    • "Earlier, Sangwan et al. (1993) reported the susceptibility of these isolates to commonly used acaricides. Recently, the acaricide resistance status in ticks collected from different parts of the country has been reported (Sachin Kumar et al. 2011, Sharma et al. 2012a, Shyma et al. 2012). "
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