Antimicrobial and Cytotoxic Activity of the Methanol Extract of Paederia foetida Linn. (Rubiaceae)

ABSTRACT The antibacterial activities of n-hexane, chloroform, ethyl acetate fractions of
methanolic extracts of the whole plants Paederia foetida (family Rubiaceae) were screened
against various pathogenic bacteria such as Bacillus cereus, Bacillus megaterium, Bacillus
subtilis, Staphylococcus aureus, Sarcina lutea, Escherichia coli, Pseudomonas aeruginosa,
Salmonella paratyphi , Salmonella typhi, Shigella boydii, Shigella dysenteriae, Vibrio mimicus,
Vibrio parahemolyticus, Candida albicans, Aspergillus niger, Sacharomyces cerevacae by ‘disc
diffusion method’. The methanol extract of the whole plants possesses no antimicrobial activity
but the ethyl acetate, chloroform and n-hexane fractions exhibited moderate to less activity
against some organisms tested compared with the standard antibiotic Kanamycin. Brine shrimp
lethality bio-assay was done using brine shrimp Nauplii and dimethyl sulfoxide as a solvent for
the methanol plant extracts of Paederia foetida. The LC50 value of methanol extract of the plant
indicated that the cytotoxicity was very significant

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    ABSTRACT: Paederia foetida L. is used as a remedy for diarrhoea and dysentery in Asia, but has not been investigated for its antidiarrhoeal properties. Antidiarrhoeal activity of 90% ethanol extract of Paederia foetida was investigated in this study using castor oil and magnesium sulphate-induced diarrhoea models in mice. The extract significantly increased the latent period of diarrhoea in both the models. In the castor oil study, the purging index (PI) value lowered in 1 h of the study at (100, 250 and 500 mg/kg) doses. The effect continued up to 6-h period only at 500 mg/kg dose. The plant notably reduced the purging index value in a dose-dependent manner in magnesium sulphate-induced diarrhoea. Paederia foetida, in general, reduced the gastrointestinal motility with barium sulphate milk both in 15- and 30-min time intervals. The extract significantly decreased the cisplatin-induced gastrointestinal motility at all doses at both time intervals. The extract also enhanced the morphine-induced reduction of motility at 500 mg/kg dose level at both time intervals. The results suggest that Paederia foetida showed antidiarrhoeal activity by inhibiting intestinal motility and justify its use in traditional medicine.
    Journal of Ethnopharmacology 05/2006; 105(1-2):125-30. · 2.94 Impact Factor


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May 26, 2014