Colorimetric assay for aromatic amines

Analytical Chemistry (Impact Factor: 5.64). 04/1976; 48(3):542-4. DOI: 10.1021/ac60367a036
Source: PubMed


Aromatic amines can be detected in the nanomole range with the reagent Fluram, with which they form stable yellow derivatives. Fluram in glacial acetic acid reacts only with aromatic amines. The reaction is complete in 10 minutes and can be performed on thin-layer (TLC) chromatograms making possible the specific measurement of aromatic amines. The yellow product can be quantitatively eluted from the TLC plates. Fluram is colorless, and the blanks are zero.

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