Colorimetric assay for aromatic amines.

Analytical Chemistry (Impact Factor: 5.7). 04/1976; 48(3):542-4. DOI: 10.1021/ac60367a036
Source: PubMed
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    ABSTRACT: A rapid method based on reverse-phase high-pressure liquid chromatography (HPLC) is described for the separation and quantitation of N-(2-propyl)-N′-phenyl-p-phenylenediamine (IPPD) and N-(1,3-dimethylbutyl)-N′-phenyl-p-phenylenediamine (DBPD) in urine. Sample preparation is based on a simple extraction procedure and analysis is carried out on a chromatographic system using a LiChrosorb RP 18 column and buffered aqueous methanol as the mobile phase. The relationship between peak heights and amount injected was linear over a range of 0.05–5 μg for both compounds. Retention times and peak heights were highly reproducible. Detection was very sensitive, allowing quantitation of 5 ng of either compound. The application of the techniques for biomonitoring body fluids as an indicator of exposure to aromatic amines is discussed.
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