Article

Cytomegalovirus- and interferon-related effects on human endothelial cells. Cytomegalovirus infection reduces upregulation of HLA class II antigen expression after treatment with interferon-gamma.

Department of Surgery, Johann Wolfgang Goethe University, Frankfurt, Germany.
Human Immunology (impact factor: 2.84). 01/1993; 35(4):230-8. DOI:10.1016/0198-8859(92)90004-7 pp.230-8
Source: PubMed

ABSTRACT Cultured human umbilical vein endothelial cells (HUVEs) were infected with human cytomegalovirus (HCMV) strain AD169. Up to 50% HUVEs proved to be positive for HCMV early nuclear antigens 24 hours after inoculation with virus. Following infection kinetics of surface expression of HLA class I and II, intercellular adhesion molecule (ICAM-1) and endothelial lymphocyte adhesion molecule (ELAM-1) on HUVEs were investigated by means of flow cytometry. A slight increase in HLA class I expression was observed, whereas expression of HLA class II (DR, DP, DQ) antigens was not induced by infection with HCMV. Furthermore, when compared with uninfected cells treated with interferon-gamma (IFN-gamma), reduced enhancement of HLA-DR expression was conspicuous in HCMV-infected cells treated with IFN-gamma. There is evidence that only a portion of HUVE is affected in its ability to upregulate HLA class II antigens. While expression of ICAM-1 was found to be enhanced between 8 and 20 hours after infection with a maximum at 12 hours after infection, no modulation of ELAM-1 was seen.

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Keywords

Cultured human umbilical vein endothelial cells
 
DQ
 
endothelial lymphocyte adhesion molecule
 
HCMV-infected cells
 
HLA class
 
HLA class II
 
HLA-DR expression
 
human cytomegalovirus
 
ICAM-1
 
infection kinetics
 
inoculation
 
intercellular adhesion molecule
 
modulation
 
positive
 
slight increase
 
surface expression
 
uninfected cells
 
upregulate HLA class II antigens
 

M Scholz