Cytomegalovirus- and interferon-related effects on human endothelial cells. Cytomegalovirus infection reduces upregulation of HLA class II antigen expression after treatment with interferon-γ

Department of Surgery, Johann Wolfgang Goethe University, Frankfurt, Germany.
Human Immunology (Impact Factor: 2.28). 01/1993; 35(4):230-8. DOI: 10.1016/0198-8859(92)90004-7
Source: PubMed

ABSTRACT Cultured human umbilical vein endothelial cells (HUVEs) were infected with human cytomegalovirus (HCMV) strain AD169. Up to 50% HUVEs proved to be positive for HCMV early nuclear antigens 24 hours after inoculation with virus. Following infection kinetics of surface expression of HLA class I and II, intercellular adhesion molecule (ICAM-1) and endothelial lymphocyte adhesion molecule (ELAM-1) on HUVEs were investigated by means of flow cytometry. A slight increase in HLA class I expression was observed, whereas expression of HLA class II (DR, DP, DQ) antigens was not induced by infection with HCMV. Furthermore, when compared with uninfected cells treated with interferon-gamma (IFN-gamma), reduced enhancement of HLA-DR expression was conspicuous in HCMV-infected cells treated with IFN-gamma. There is evidence that only a portion of HUVE is affected in its ability to upregulate HLA class II antigens. While expression of ICAM-1 was found to be enhanced between 8 and 20 hours after infection with a maximum at 12 hours after infection, no modulation of ELAM-1 was seen.

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    • "sion in infected endothelial cells (Scholz et al., 1992; Sedmak et al., 1994). Though IFN␤ may play some role in HCMV-mediated inhibition of endothelial cell MHC class II expression, direct infection also appears to result in inhibition of MHC class II expression (Scholz et al., 1992; Sedmak et al., 1995, 1994). Long-term infection of M␾ with HCMV also results in decreased MHC class II expression (Fish et al., 1996). "
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