Silymarin protects against paracetamol-induced lipid peroxidation and liver damage. J Appl Toxicol

Departamento de Farmacologia y Toxicologia, Instituto Politecnico Nacional, Mexico D.F.
Journal of Applied Toxicology (Impact Factor: 3.17). 12/1992; 12(6):439-42. DOI: 10.1002/jat.2550120613
Source: PubMed

ABSTRACT The effect of silymarin on liver damage induced by acetaminophen (APAP) intoxication was studied. Wistar male rats pretreated (72 h) with 3-methylcholanthrene (3-MC) (20 mg kg-1 body wt. i.p.) were divided into three groups: animals in group 1 were treated with acetaminophen (APAP) (500 mg kg-1 body wt. p.o.), group 2 consisted of animals that received APAP plus silymarin (200 mg kg-1 body wt. p.o.) 24 h before APAP, and rats in group 3 (control) received the equivalent amount of the vehicles. Animals were sacrificed at different times after APAP administration. Reduced glutathione (GSH), lipid peroxidation and glycogen were measured in liver and alkaline phosphatase (AP), gamma-glutamyl transpeptidase (GGTP) and glutamic pyruvic transaminase (GPT) activities were measured in serum. After APAP intoxication, GSH and glycogen decreased very fast (1 h) and remained low for 6 h. Lipid peroxidation increased three times over the control 4 and 6 h after APAP treatment. Enzyme activities increased 18 h after intoxication. In the group receiving APAP plus silymarin, levels of lipid peroxidation and serum enzyme activities remained within the control values at any time studied. The fall in GSH was not prevented by silymarin, but glycogen was restored at 18 h. It was concluded that silymarin can protect against APAP intoxication through its antioxidant properties, possibly acting as a free-radical scavenger.

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    • "Silymarin can block the binding of potentially hepatocellular toxins to the outer surface of the cells and directly relief the hepatocytes (Campos et al., 1989; Muriel et al., 1992). Silymarin, as a strong free radical scavenger, has attracted intensive attention for its effect to increase the formation of glutathione in hepatocytes (Sharma et al., 2008). "
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    • "The rats were divided evenly into five groups of 8 rats and treated intraperitoneally with CCl 4 (8% CCl 4 /corn oil, 1 mL/kg body weight (BW) twice a week, Monday and Thursday) for 8 weeks, as described by Hernández-Muoz et al. [23], with some modifications. At the same time, the rats were treated with various dosages of OGE (0–0.04 g/kg BW), or silymarin orally (0.2 g/kg BW, four times a week, Tuesday, Wednesday, Friday, and Saturday) [24] [25]. The control rats were treated with corn oil (1 mL/kg BW) and fed a normal diet. "
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