Article

Prothymosin alpha expression occurs during G1 in proliferating B or T lymphocytes.

Department of Medicine, Cornell University Medical College, New York, NY 10021.
Biochemical and Biophysical Research Communications (Impact Factor: 2.28). 07/1992; 185(3):953-9. DOI: 10.1016/0006-291X(92)91719-7
Source: PubMed

ABSTRACT To gain insight into possible functions for prothymosin alpha in the proliferative cycle of lymphocytes, we examined the kinetics of prothymosin alpha mRNA expression in mitogen stimulated murine lymphocytes. This mRNA increases after mitogen stimulation, peaking in mid G1. This kinetics is compatible with induction of the prothymosin alpha gene by the c-myc protein (Eilers, M., Schirm, S. and Bishop, J.M. (1991) EMBO J., 10, 133-141). Thus, although prothymosin alpha mRNA is found throughout the cell cycle, the elevated expression in G1 may be associated with an increased requirement for prothymosin alpha during the G1/S transition or the S phase of the cell cycle.

1 Follower
 · 
50 Views
  • [Show abstract] [Hide abstract]
    ABSTRACT: Prothymosin α (ProTα) is a 12.5-kDa, highly acidic protein widely distributed in different cell types expressed intracellularly and extracellularly. ProTα does not contain a secretion-signal sequence and is released by a nonclassical secretory pathway with a cargo protein. New findings on the extracellular function of ProTα have yielded exciting insights into the cytokine-like functions of this host protein that stimulates type I interferon via Toll-like receptor 4. Here, we discuss the intracellular function of ProTα, how new findings of cytokine-like activities of ProTα aid our understanding of mechanisms that direct ProTα functions, and the potential application of these new insights to the development of immunotherapies.
    Future medicinal chemistry 07/2011; 3(9):1199-208. DOI:10.4155/fmc.11.72 · 4.00 Impact Factor
  • Clinical Immunology and Immunopathology 12/1992; 65(3):195-200. DOI:10.1016/0090-1229(92)90146-F
  • [Show abstract] [Hide abstract]
    ABSTRACT: A genomic clone encoding prothymosin alpha (gene symbol: PTMA), a nuclear-targeted protein associated with cell proliferation, was isolated and the 5'-regulatory region subcloned and sequenced. Because of previously reported discrepancies between several cDNA clones and a genomic clone for prothymosin alpha, we determined the sequence of the first exon and of a 1.7-kb region 5' to the first exon. The sequence of the genomic clone reported here corresponds to the published cDNA sequences, suggesting that the previously noted discrepancies may be due to genetic polymorphism in this region. In addition, our sequence data extend the known 5'-upstream sequence by an additional 1.5 kb allowing the identification of numerous, potential cis-acting regulatory sites. This 5'-flanking cloned probe permitted us to localize the prothymosin gene to chromosome 2 in humans.
    Human Genetics 03/1993; 90(6):629-34. DOI:10.1007/BF00202480 · 4.52 Impact Factor