Article

A cultura de células em 3 dimensões e a sua aplicação em estudos relacionados a formação do lúmen

Naturalia 01/2011; 34:1-20.

ABSTRACT Cell culture is characterized by maintaining live cells in the laboratory regardless of the
organism in which they originated. This technique has contributed to better
understanding of molecular cell mechanisms, permitting important scientific advances,
for example, concerning vaccine production and tumor cell biology. Three-dimensional
(3D) cell culture initially derived from commonly used cell cultures (monolayer cell
cultures). As a particularity, a 3D cell culture permits cells to explore the three
dimensions of the space thereby increasing cell-cell interactions, as well as interaction
with the environment. When grown in this system, cells form structures known as
multicelullar spheroids. The interior of these spheroids present cell heterogeneity,
microenvironment formation and different exposure to factors, such as nutrients and
oxygen. Owing to the fact that these characteristics are very similar to those of in vivo
avascular tumors, 3D cell culture advanced in various research lines, thus becoming a
widely used model in radiology and chemotherapy essays. In studies related to breast
cancer biology, spheroids are becoming widely used in the aim to comprehend luminal
space morphogenesis.

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