Biosynthesis, characterization and antimicrobial studies of AgNPs extract from bacopa monniera whole plant
Journal Article: Digest Journal of Nanomaterials and Biostructures (impact factor: 1.75). 01/2011; 6:135-142.
Abstract
Reliable and eco-friendly process for synthesis of metallic nanoparticles is an important
step in the field of the nanotechnology. One of the options to achieve this objective is to
use biological systems. Here we report the ethanolic extract of B. monniera to produce
silver nanoparticles by reduction of AgNO3. It was observed that the synthesis process was
quite rapid and silver nanoparticles were formed with in minutes of silver ion coming in
contact with the plant filtrate. UV-visible spectrum of the aqueous medium containing
silver ions demonstrated a peak at 436 nm corresponding to the plasmon absorbance of
silver nanoparticles. X-Ray diffraction (XRD) analysis reveals the FCC structure of silver
nanoparticles. Energy dispersive X-ray analysis (EDX) evidences the presence of silver
nanoparticles in the aquatic solution of leaf extract. FTIR analysis of the nanoparticles
indicated the presence of proteins, which may be acting as capping agents around the
nanoparticles. From transmission electron microscopy (TEM) analysis, the size of the
silver nanoparticles was measured (10-30 nm). Further the antimicrobial activity of
synthesized particles showed effective inhibitory activity.
step in the field of the nanotechnology. One of the options to achieve this objective is to
use biological systems. Here we report the ethanolic extract of B. monniera to produce
silver nanoparticles by reduction of AgNO3. It was observed that the synthesis process was
quite rapid and silver nanoparticles were formed with in minutes of silver ion coming in
contact with the plant filtrate. UV-visible spectrum of the aqueous medium containing
silver ions demonstrated a peak at 436 nm corresponding to the plasmon absorbance of
silver nanoparticles. X-Ray diffraction (XRD) analysis reveals the FCC structure of silver
nanoparticles. Energy dispersive X-ray analysis (EDX) evidences the presence of silver
nanoparticles in the aquatic solution of leaf extract. FTIR analysis of the nanoparticles
indicated the presence of proteins, which may be acting as capping agents around the
nanoparticles. From transmission electron microscopy (TEM) analysis, the size of the
silver nanoparticles was measured (10-30 nm). Further the antimicrobial activity of
synthesized particles showed effective inhibitory activity.
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