Solid Phase Enzyme Immunoassays for the Quantification of Serum Amyloid P (SAP) and Complement Component 3 (C3) Proteins in Acute-Phase Mouse Sera

National Institute for Biological Standards and Control, Division of Endocrinology, South Mimms, Potters Bar, Herts, United Kingdom.
Hormone and Metabolic Research (Impact Factor: 2.12). 09/1992; 24(8):371-4. DOI: 10.1055/s-2007-1003338
Source: PubMed


Simple, reliable and sensitive enzyme immunoassays have been developed for the quantification of the mouse acute-phase SAP and C3 proteins. The ELISA systems were validated using sera from mice injected with S. dysenteriae endotoxin, and detected 500 pg protein/ml. The assays use 96-well microtitre plates permitting rapid processing of a large number of samples.

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