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    ABSTRACT: Pollen tubes in the styles of the tomato and of other flowering plants can be observed by using the following technic. Styles are fixed in formalin-acetic-80% alcohol (1:1:8) and cleared and softened in a strong (8 N) sodium hydroxide solution. Staining is accomplished in a 0.1% solution of water-soluble aniline blue dye dissolved in 0.1 N, K3PO4. The styles are smeared or are observed whole under a conventional or dissecting microscope by direct illumination with ultraviolet light of a wavelength of about 356 m°. Observations are made in a darkened room. Under these conditions callose fluoresces bright yellow-green and contrasts strongly with the bluish or grayish fluorescence of the stylar tissue. The pollen tubes are outlined by a callose lining and irregularly spaced callose plugs.
    Stain technology 06/1959; 34(3):125-8.
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    ABSTRACT: This study was undertaken in order to understand thebehaviour of airborne pollen grains, namely therelationship between their concentrations andconcomitant meteorological parameters, at differentaltitudes (ground level, 200, 400 and 600 m) of thelower atmosphere and its daily variations over onesite (the experimental farm of the University ofLen). The experimental design involved a tetheredballoon (2.25 m3), an ADAS remote meteorologicalstation and an original radio controlled three headedpollen and spore sampler (called GABIS) using theRotorod design. Each head sampled a differentaltitude, while two control samples were taken atground level. Twenty-one takeoffs could be realized inthe 5 weeks period between end of May and end of June1997. Sampling was done early in the morning and sampling time at each altitudewas of 15 minutes. Results show that 45 differenttypes of pollen grains could be collected at this timeof the year and that significant variations could beobserved in the behaviour of the pollen cloud on a dayto day basis, probably because of differentmeteorological situations. Contrary to what isgenerally believed, pollen was in most cases moreabundant at higher altitude – on average by 30% ascompared to ground level – making evident anaerobiological layer of transport at about 500 m aboveground. This was especially the case for trees (Quercus and Castanea). The atmosphere MixingRatio was the most explicative factor at 200 m, whiletemperature dominated significance analysis at both400 and 600 m.
    Aerobiologia 01/2000; 16(2):187-191. · 1.20 Impact Factor
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    ABSTRACT: In the first section, various techniques are described for preparing pollen for study by scanning electron microscopy. The techniques include acetolysis, acid and basic hydrolysis, ultrasonic splitting of the exine, mounting and sectioning. The effects of the different treatments on the pollen are compared and summarized in a table which contains appropriate cross references to the illustrations provided. The second part is essentially a review of information obtained by these various techniques in the study of the exine and apertures of pollen grains of woody members of the Saxifragaceae.RésuméL'auteur décrit, dans une premiére partie, les techniques de préparation du pollen pour les observations au MEB: techniques diverses relatives à la solubilisation de l'intine cellulosique (fossilisation artificielle) et à l'obtention de surfaces de rupture de l'exine par les ultrasons; techniques relatives à l'observation du pollen ‘frais’ avec ou sons coupes semi-fines et enfin techniques de montage du pollen traité, sur lamelle, pour le MEB. Il compare les effets des différents traitements physico-chimiques sur le pollen dans un tableau récapitulatif valable aussi bien pour le microscope photonique que pour le MEB. Les clichés accompagnant le texte illustrent la presque totalité des techniques décrites dans le tableau. Dans une seconde partie, il rassemble les informations apportées par ces techniques variées pour l'étude de l'exine et des apertures des grains de pollen de quelques Saxifragacées ligneuses.ZusammenfassungIm ersten Abschnitt werden Methoden für das Präparien von Pollen beschrieben, der im Rasterelektronenmikroskop untersucht wird. Die Beschreibung umfasst das Präparieren frischer und azetolisierter Körner, die Ultraschallwellenspaltung der äusseren Pollenhaut, die Herstellung von Dünnschnitten und das Montieren. Die Ergebnisse dieser physikalish-chemischer Pollenbehandlungen werden in einer Tabelle zusammengefasst und verglichen, und durch beigefügte Illustrationen erläutert. In einer Zusammenstellung, welche den zweiten Teil dieses Artiekls bildet, werden die Ergebnisse der verschiedenen Verfahren angeführt, welche zur Untersuchung der äusseren Pollenhaut und ihrer Aperturen bei den holzigen Saxifragaceen verwendet wurden.
    Micron (1969) 01/1971; 3(1):1-31. · 2.06 Impact Factor

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