Induction of heme oxygenase by delta 12-prostaglandin J2 in porcine aortic endothelial cells.

Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
Prostaglandins 03/1992; 43(2):121-31. DOI: 10.1016/0090-6980(92)90081-4
Source: PubMed

ABSTRACT delta 12-Prostaglandin (PG)J2 stimulated the synthesis of a 31,000-dalton protein (termed p31) and the induction of cellular heme oxygenase activity in porcine aortic endothelial cells. A good correlation was observed between the time courses and dose dependencies of the induction of p31 synthesis and that of heme oxygenase activity by delta 12-PGJ2. Hemin, a known inducer of heme oxygenase, also induced p31 synthesis as well as heme oxygenase activity in the cells. On two-dimensional gel electrophoresis, p31 induced by delta 12-PGJ2 exhibited an isoelectric point of 5.4, which coincided exactly with that induced by hemin. These results indicate that the p31 induced by delta 12-PGJ2 in porcine aortic endothelial cells is heme oxygenase.