Induction of heme oxygenase by delta 12-prostaglandin J2 in porcine aortic endothelial cells.
ABSTRACT delta 12-Prostaglandin (PG)J2 stimulated the synthesis of a 31,000-dalton protein (termed p31) and the induction of cellular heme oxygenase activity in porcine aortic endothelial cells. A good correlation was observed between the time courses and dose dependencies of the induction of p31 synthesis and that of heme oxygenase activity by delta 12-PGJ2. Hemin, a known inducer of heme oxygenase, also induced p31 synthesis as well as heme oxygenase activity in the cells. On two-dimensional gel electrophoresis, p31 induced by delta 12-PGJ2 exhibited an isoelectric point of 5.4, which coincided exactly with that induced by hemin. These results indicate that the p31 induced by delta 12-PGJ2 in porcine aortic endothelial cells is heme oxygenase.
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Arteriosclerosis Thrombosis and Vascular Biology 11/2001; 21(11):1846-1851. DOI:10.1161/hq1101.098488 · 5.53 Impact Factor
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ABSTRACT: Prostaglandin (PG) A2 (PGA2) and Δ12-PGJ2 have potent antiproliferative activity on various tumor cell growths in vitro. In this study, we investigated the mechanism of PGA2/Δ12-PGJ2-mediated apoptosis, including intracellular apoptosis-related genes in human hepatocarcinoma Hep3B cells. Hep3B cells treated with PGA2/Δ12-PGJ2 showed that a time-dependent DNA fragmentation characterized by marked apoptosis and the elevation of c-myc mRNA expression. In proportion to the increased c-myc gene transcription, heat shock protein 70 (hsp70) mRNA was induced from 1 to 24 h after PGA2/Δ12-PGJ2 treatment. The transfection of c-myc antisense oligomers in Hep3B cells significantly delayed the induction of HSP70 expression and blocked formation of DNA fragmentation by PGA2/Δ12-PGJ2. Moreover, overexpressed HSP70 showed an increased resistance to apoptosis by PGA2/Δ12-PGJ2 treatment. These results demonstrated that the decreased survival in response to PGA2/Δ12-PGJ2 was causally related to the amount of c-myc and the induction of c-myc regulated the elevation of HSP70 which have been known to correlate with a resistance to apoptosis.Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 11/1998; 1448(1):115-125. DOI:10.1016/S0167-4889(98)00113-X · 5.30 Impact Factor