Anti-infective effect of poly-β1-6-glucotriosyl-β1-3-glucopyranose glucan in vivo
ABSTRACT Mice challenged with Escherichia coli or Staphylococcus aureus were protected against lethal peritonitis by the intravenous administration of 10 micrograms of poly-beta 1-6-glucotriosyl-beta 1-3-glucopyranose (PGG) glucan per animal 4 to 6 h prior to bacterial challenge. Subsequent studies with the rat model for intra-abdominal sepsis indicated that intramuscular doses of 10 to 100 micrograms per animal 24 and 4 h prior to surgical implantation of the bacterial inoculum reduced the early mortality associated with the peritonitis phase of this experimental disease process. Quantitative cultures of blood obtained from challenged rats showed that significantly fewer organisms were present in the blood of PGG glucan-treated animals than in that of untreated animals. Quantitative studies of leukocytes of rats and mice following a single injection of PGG glucan showed a modest transient increase in the total leukocyte count. The possible mechanisms by which protection occurs in the animal model system are discussed.
Full-textDOI: · Available from: Gary R Ostroff, Jul 16, 2014
- SourceAvailable from: Ming-Tang Chiou
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- "products of G. lucidum. The b-glucans have been reported to inhibit tumour formation (Ohno et al. 1995), enhance defence against bacterial challenge (Onderdonk et al. 1992; Babineau et al. 1994), and increase growth performance (Schoenherr et al. 1994) in pigs. Glucans from a variety of yeast cell walls have been shown to stimulate both specific and non-specific immune responses (Dritz et al. 1995). "
ABSTRACT: The purpose of this study was to test fermentation, for its products of a Chinese medicinal mushroom, Ganoderma lucidum, cultured by submerged fermentation for its effect on growth performance and immunocompetence in weanling piglets. In Experiment 1, 72 weanling piglets were allotted to one of four treatments receiving these fermentation products (GLF, expressed as amount of beta-glucans) at 0 (control), 50, 100, and 150 mg/kg feed for 4 weeks. The results showed that at a supplementation level of 50 mg/kg feed, GLF caused the best growth performance, the highest pseudorabies antibody titre, and a decrease of blood glucose level. It was also demonstrated that GLF up-regulated the cell-mediated immune response related cytokines (IL-2, IFN-gamma, and TNF-alpha) expression in different lymphoid tissues. After challenging with porcine circovirus (PCV) type 2 (Experiment 2), a supplementation with 50 mg GLF per kg feed also inhibited PCV-2 virus amplification, and ameliorated lymphocyte depletion in different lymphoid tissues. Conclusively, feed supplemented with GLF at 50 mg/kg could be beneficial to counteract the physiological stress in weanling piglets.Archives of animal nutrition 03/2008; 62(1):22-32. DOI:10.1080/17450390701780201 · 0.89 Impact Factor
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- "Several studies conducted in the past decade have showed that it inhibits tumor development, enhances defense against bacterial, viral, fungal, parasitic challenge (Onderdonk et al., 1992; Kernoddle et al., 1998), activates macrophages (Cleary et al., 1999; Vetvicka and Yvin, 2004), induces production of cytokines (Soltys and Quinn, 1999; Engstad et al., 2002), nitric oxide (NO), arachidonic acid metabolites (Ljungman et al., 1998) increases hematopoesis, exerts radioprotective effects, improves wound healing by inducing the macrophage release of wound growth factors (Wei et al., 2002) and lower serum lipids (Nicolosi et al., 1999). Several mechanisms were proposed for the protective effect of ␤-glucan, one of them is related to antioxidant capacity of the molecule (Babincova et al., 2002; Krizkova et al., 2003; Sener et al., 2005a). "
ABSTRACT: In this study, we investigated the protective effect of β-glucan against nicotine induced oxidative damage in urinary bladder and kidney tissues. Wistar albino rats were injected i.p. with nicotine hydrogen bitartarate (0.6mg/kg daily for 21 days) or saline. β-Glucan (50mg/kg, p.o.) was administered alone or with nicotine injections for 21 days. After decapitation, the urinary bladder and kidney tissues were taken for the measurement of malondialdehyde (MDA) and glutathione (GSH) levels, and myeloperoxidase (MPO) activity. Tissue samples were also examined histologically. In serum samples MDA, GSH, BUN, creatinine, TNF-α levels and LDH activity were analyzed. Chronic nicotine administration caused a significant decrease in GSH levels and increases in MDA levels and MPO activity in kidney and bladder tissues, suggesting oxidative organ damage, which was also histologically verified. Furthermore, β-glucan restored the reduced GSH levels, while it significantly decreased MDA levels and MPO activity. Renal function tests, LDH and TNF-α levels, which were increased significantly due to nicotine administration, were decreased with β-glucan treatment. The present data suggest that β-glucan supplementation effectively counteracts the chronic nicotine toxicity and attenuates oxidative damage of bladder and kidney tissues possibly by its antioxidant effects.Environmental Toxicology and Pharmacology 01/2007; 23(1):25-32. DOI:10.1016/j.etap.2006.06.003 · 2.08 Impact Factor