Anti-infective effect of poly-β1-6-glucotriosyl-β1-3-glucopyranose glucan in vivo

Department of Pathology, Channing Laboratory, Brigham and Women's Hospital, Boston, Massachusetts.
Infection and Immunity (Impact Factor: 4.16). 05/1992; 60(4):1642-7.
Source: PubMed

ABSTRACT Mice challenged with Escherichia coli or Staphylococcus aureus were protected against lethal peritonitis by the intravenous administration of 10 micrograms of poly-beta 1-6-glucotriosyl-beta 1-3-glucopyranose (PGG) glucan per animal 4 to 6 h prior to bacterial challenge. Subsequent studies with the rat model for intra-abdominal sepsis indicated that intramuscular doses of 10 to 100 micrograms per animal 24 and 4 h prior to surgical implantation of the bacterial inoculum reduced the early mortality associated with the peritonitis phase of this experimental disease process. Quantitative cultures of blood obtained from challenged rats showed that significantly fewer organisms were present in the blood of PGG glucan-treated animals than in that of untreated animals. Quantitative studies of leukocytes of rats and mice following a single injection of PGG glucan showed a modest transient increase in the total leukocyte count. The possible mechanisms by which protection occurs in the animal model system are discussed.

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Available from: Gary R Ostroff, Jul 16, 2014
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    • "products of G. lucidum. The b-glucans have been reported to inhibit tumour formation (Ohno et al. 1995), enhance defence against bacterial challenge (Onderdonk et al. 1992; Babineau et al. 1994), and increase growth performance (Schoenherr et al. 1994) in pigs. Glucans from a variety of yeast cell walls have been shown to stimulate both specific and non-specific immune responses (Dritz et al. 1995). "
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    • "Several studies conducted in the past decade have showed that it inhibits tumor development, enhances defense against bacterial, viral, fungal, parasitic challenge (Onderdonk et al., 1992; Kernoddle et al., 1998), activates macrophages (Cleary et al., 1999; Vetvicka and Yvin, 2004), induces production of cytokines (Soltys and Quinn, 1999; Engstad et al., 2002), nitric oxide (NO), arachidonic acid metabolites (Ljungman et al., 1998) increases hematopoesis, exerts radioprotective effects, improves wound healing by inducing the macrophage release of wound growth factors (Wei et al., 2002) and lower serum lipids (Nicolosi et al., 1999). Several mechanisms were proposed for the protective effect of ␤-glucan, one of them is related to antioxidant capacity of the molecule (Babincova et al., 2002; Krizkova et al., 2003; Sener et al., 2005a). "
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