Antimicrobial activity of pomegranate rind peel extracts

African journal of microbiology research (Impact Factor: 0.54). 10/2011; 4(22):3664-3668.


The pomegranate, Punica granatum L., is an ancient, mystical, unique fruit borne on a small, long-living tree cultivated throughout the Mediterranean region. Pomegranate is used in several systems of medicine for a variety of ailments. The synergistic action of the pomegranate constituents appears to be superior to that of single constituents. P. garantum, have been reported to have antimicrobial activity against a range of Gram positive and negative bacteria. Pomegranate formulations containing ferrous salts have enhanced although on short-term. The aim of this experiment is to determine the
antimicrobial activities of combinations of pomegranate rind extract with range of metal salts with the addition of vitamin C. Phytochemical analyses was made to determine the active inhibitors in rind extract, including phenolics and flavonoids.

Download full-text


Available from: Ahmed E Abdel Moneim, Oct 05, 2015
125 Reads
  • Source
    • "Our study was focused on the protective effect of pomegranate juice against CCl4-induced reproductive toxicity in male rats. Pomegranate has a protective effect due to its active ingredients like tannins [23,24], phenolic acids [25], estrogenic flavonoids [26] and conjugated fatty acids [27], these compounds are found in substantial amounts in the peels, juice and seed oil of the pomegranate fruits [28]. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Pomegranate fruit has been extensively used as a natural medicine in many cultures. The present study was aimed at evaluating the protective effects of pomegranate (Punica granatum) juice against carbon tetrachloride (CCl4)-induced oxidative stress and testes injury in adult Wistar rats. Twenty eight Wistar albino male rats were divided equally into 4 groups for the assessment of protective potential of pomegranate juice. Rats of group I (control) received only vehicles and had free access to food and water. Rats of groups II and IV were treated with CCl4 (2 ml/kg bwt) via the intraperitoneal route once a week for ten weeks. The pomegranate juice was supplemented via drinking water 2 weeks before and concurrent with CCl4 treatment to group IV. Group III was supplemented with pomegranate juice for twelve weeks. The protective effects of pomegranate on serum sex hormones, oxidative markers, activities of antioxidant enzymes and histopathology of testes were determined in CCl4-induced reproductive toxicity in rats. Pomegranate juice showed significant elevation in testosterone, luteinizing hormone (LH) and follicle stimulating hormone (FSH) those depleted by the injection of CCl4. Activity levels of endogenous testesticular antioxidant enzymes; superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and glutathione reductase (GR) and glutathione (GSH) contents were increased while lipid peroxidation (LPO) and nitric oxide (NO) were decreased with pomegranate juice. Moreover, degeneration of germ and Leydig cells along with deformities in spermatogenesis induced after CCl4 injections were restored with the treatment of pomegranate juice. The results clearly demonstrated that pomegranate juice augments the antioxidant defense mechanism against carbon tetrachloride-induced reproductive toxicity and provides evidence that it may have a therapeutic role in free radical mediated diseases.
    BMC Complementary and Alternative Medicine 05/2014; 14(1):164. DOI:10.1186/1472-6882-14-164 · 2.02 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The purpose of this study was to evaluate the effect of pomegranate (Punica granatum) in inhibiting and reversing the nephrotoxicity of carbon tetrachloride, a potent oxidative stress inducer which induces cellular kidney damage. Rats were intraperitoneally injected with carbon tetrachloride (2 mL/kg body weight) which produced severe renal tissue damage, as demonstrated by decreased uric acid and dramatic elevation of urea and creatinine. In addition, carbon tetrachloride injection caused oxidative stress in rats, as evidenced by increased lipid peroxidation and nitrite/nitrate (NO( x )) concentrations in the renal tissue, along with a remarkable reduction in superoxide dismutase, catalase, glutathione transferase, glutathione reductase, glutathione peroxidase activities and glutathione content. We suggested that pomegranate juice was able to elevate the antioxidant defense system, clean up free radicals, lessen oxidative damages and protect the kidney against carbon tetrachloride-induced toxicity, thus having a potential protective effect.
    Journal of physiology and biochemistry 11/2012; 69(3). DOI:10.1007/s13105-012-0218-3 · 1.97 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Punica granatum are utilized by local people as the part of their meal. Peoples supposed to consume Punica granatum seed and their peel was thrown as waste. Present study was designed to evaluate the antibacterial activity of the DMSO, ethanol and methanol extracts of Egyptian and Taif cultivars of Punica granatum peel and seeds extracts against five opportunistic pathogens namely Staphylococcus aureus, Pseudomonas sp. and Bacillus sp. (as a gram positive bacteria) and Escherichia coli and Aeromonas hydrophila (as a gram negative bacteria). The antimicrobial activity of plant peels and seeds extracts were compared with that of Streptomycin, as reference antibiotics. Evaluations were based on the zone of inhibition using agar well diffusion assay. The inhibitory activity was found to be dose dependent. The methanol extract of the peels from the two cultivars at 15 mg/ml dosage were found to be active antimicrobials against all the test microorganisms. While, the maximum antimicrobial activity for the seeds extracts were reported at 60 mg/ml. Escherichia coli was reported to have significant susceptibility against most of the extract followed by Staphylococcus aureus and Pseudomonas sp. Additionally, we evaluated the effect of the peels and seeds extract on the DNA damage of the bacterial genome using the repetitive element PCR analysis technique (Rep-PCR). The Rep-PCR results significantly showed that there are a polymorphic numbers of genetic bands, which were found in E. coli treated bacteria and absent in the untreated bacteria. This study indicates that aqueous DMSO, methanol and ethanol extracts of peels and seeds of the two cultivars of Punica granatum may be utilizes as a potential source of antimicrobial and mutagen agents. Key words: Punica granatum, antibacterial activity, Peels and seeds extracts and Rep-PCR.
    01/2015; 3(2):012-017. DOI:10.7324/JABB.2015.3203