A method for small scale genomic DNA isolation from chickpea (Cicer arietinum L.) suitable for molecular marker analysis

Indian Journal of Science and Technology 01/2010; 3:1214-1217.

ABSTRACT We describe a DNA isolation procedure for chickpea (Cicer arietinum L.) which is rapid and less expensive without
involving ultra centrifugation or column purification steps. DNA preparation obtained from the present study was
essentially suitable for PCR analysis which is one of the key steps in crop improvement programme through marker
development and genetic engineering techniques. The yield of DNA ranged from 0.595- 5.550 μg/ml and the purity
ratio was between 1.025- 2.010 indicating minimum levels of contaminating metabolites. The present protocol offers as
a reliable, and consistent DNA isolation method for chickpea that yields large amount of pure & intact DNA.

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