Effects of inoculation with Phanerochaete chrysosporium on remediation of pentachlorophenol-contaminated soil waste by composting

PROCESS BIOCHEMISTRY (Impact Factor: 2.52). 06/2011; 46(6):1285-1291. DOI: 10.1016/j.procbio.2011.02.018

ABSTRACT The effect of inoculation with Phanerochaete chrysosporium (P. chrysosporium) during different fermentation phases on remediation of pentachlorophenol (PCP)-contaminated soil waste (PSW) was investigated over 60 days. This was accomplished by evaluating physico-chemical and biochemical properties of composts, as well as bacterial community composition using denaturing gradient gel electrophoresis (DGGE). Results showed that the inoculations could significantly enhance composting efficiency and PCP removal. The best degree of maturity and highest PCP removal occurred in Run C (inoculation during the second fermentation phase) were compared with Runs A (control treatment) and B (inoculation during the first fermentation phase). A positive effect on production of manganese peroxidase (MnP) and lignin peroxidase (LiP) was found in inoculated runs, especially in Run C, while the production of laccase (Lac) was limited by P. chrysosporium inoculants. As a result of DGGE analysis, the compost bacterial community composition was altered by different inoculations, as indicated by the differences between the final composts. This study highlights the different effects of the inoculations on remediation performance of PSW. The inoculation during the second fermentation phase is more effective than that during the first fermentation phase

  • [Show abstract] [Hide abstract]
    ABSTRACT: The purpose of our study was 2-fold: (i) to evaluate the effect of dominant electron acceptor [either aerobic, methanogenic, or sulfate-reducing slurry bioreactor (SB)] and biostimulation with sucrose on lindane removal from heavy soil and (ii) to assess the effect of the type of combined environments [partially aerated methanogenic (PAM) and simultaneous methanogenic-sulfate reducing (M-SR)] and addition of silicone oil as solvent on lindane removal from a clayish agricultural soil with high levels of organic matter.In the first experiment, the main effect of electron acceptor was significant (pA≫M SBs. On the other hand, co-substrate sucrose was not significant (p=0.67). Yet, the interaction was moderately significant (p
    Journal of Biotechnology 11/2012; 47(11). DOI:10.1016/j.procbio.2011.10.013 · 2.88 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The white-rot fungus Phanerochaete chrysosporium has been widely used for the treatment of waste streams containing heavy metals and toxic organic pollutants. The development of fungal-based treatment technologies requires detailed knowledge of the relationship between bulk water quality and the physiological responses of fungi. A noninvasive microtest technique was used to quantify real-time changes in proton, oxygen, and cadmium ion fluxes following the exposure of P. chrysosporium to environmental toxic (2,4-dichlorophenol and cadmium). Significant changes in H(+) and O(2) flux occurred after exposure to 10 mg/L 2,4-dichlorophenol and 0.1 mM cadmium. Cd(2+) flux decreased with time. Reactive oxygen species formation and antioxidant levels increased after cadmium treatment. Superoxide dismutase activity correlated well with malondialdehyde levels (r(2) = 0.964) at low cadmium concentrations. However, this correlation diminished and malondialdehyde levels significantly increased at the highest cadmium concentration tested. Real-time microscale signatures of H(+), O(2), and Cd(2+) fluxes coupled with oxidative stress analysis can improve our understanding of the physiological responses of P. chrysosporium to toxic pollutants and provide useful information for the development of fungal-based technologies to improve the treatment of wastes cocontaminated with heavy metals and organic pollutants.
    Environmental Science & Technology 06/2012; 46(14):7818-25. DOI:10.1021/es301006j · 5.48 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Soil-borne plant pathogens are responsible for causing many crop plant diseases, resulting in significant economic losses. Compost application to agricultural fields is an excellent natural approach, which can be taken to fight against plant pathogens. The application of organic waste products is also an environmentally friendly alternative to chemical use, which unfortunately is the most common approach in agriculture today. This review analyses pioneering and recent compost research, and also the mechanisms and mode of action of compost microbial communities for reducing the activity of plant pathogens in agricultural crops. In addition, an approach for improving the quality of composts through the microbial communities already present in the compost is presented. Future agricultural practices will almost definitely require integrated research strategies to help combat plant diseases.
    Waste Management 12/2013; 34(3). DOI:10.1016/j.wasman.2013.11.012 · 3.16 Impact Factor

Full-text (2 Sources)

Available from
May 31, 2014