Evaluation of Antihyperglycemic Activity of Citrus limetta Fruit Peel in Streptozotocin-Induced Diabetic Rats

Department of Pharmaceutical Technology, Jadavpur University, Kolkata, West Bengal 700032, India.
ISRN Endocrinology 07/2011; 2011. DOI: 10.5402/2011/869273
Source: PubMed

ABSTRACT The present paper aims to evaluate antihyperglycemic activity of methanol extract of Citrus limetta fruit peel (MECL) in
streptozotocin-induced (STZ; 65mg/kg b.w.) diabetic rats. Three days after STZ induction, diabetic rats received MECL orally
at 200 and 400 mg kg−1 body weight daily for 15 days. Glibenclamide (0.5mg kg−1 p. o.) was used as reference drug. Blood glucose
levels were measured on 0th, 4th, 8th, and 15th days of study. Serum biochemical parameters namely, SGOT, SGPT and ALP
were estimated. The TBARS and GSH levels of pancreas, kidney, and liver were determined. MECL significantly (P < 0.001) and
dose dependently normalized blood glucose levels and serumbiochemical parameters, decreased lipid peroxidation, and recovered
GSH as compared to those of STZ control. The present paper infers that in STZ-induced diabetic Wistar rats, C. limetta fruit peel
demonstrated a potential antihyperglycemic effect which may be attributed to its antioxidant property.

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Article: Evaluation of Antihyperglycemic Activity of Citrus limetta Fruit Peel in Streptozotocin-Induced Diabetic Rats

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    • "A 2009 study examining extracts from a Korean Citrus fruit called Dangyuja (Citrus grandis) found that it holds great potential for controlling blood glucose levels in diabetic patients [8] . Citrus limetta fruit peel demonstrated a potential anti-hyperglycemic effect in streptozotocin induced diabetic rats [9] . "
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    ABSTRACT: Objective: To investigate the therapeutic effects of methanol extract of Citrus macroptera fruit in α-amylase inhibitory activity (in vitro) and hypoglycemic activity in normal and g Mluocnotsre. induced hyperglycemic rats (in vivo). Methods: Fruits of Citrus macroptera without rind was extracted with pure methanol following cacotlidv ietyx,t raancdti honyp aongldy cteemsteicd effofer cpt riens neonrcme aol fr apths yatnodch gelmuciocsael icnodnuscteitdu ehnytpse, rαgl-ycaemmyilca srea tsin. hibitory Results: Presence of saponin, steroid and terpenoid were identified in the extract. The results smhgo/wmeLd] tahsa tc ofrmupita erexdtr atoc t ahcaadrb mosoed.e Mraotere αov-earm ayt la5s0e0 mingh/ikbgit oarnyd a 1c t0i0v0i tym g[I/Ck5g0 vdaolsuees= f(3ru.6i3t 8e依x0t.r1a9c0t) sraigtsn iafisc acnotmlyp a(Pre<d0 .t0o5 galnibde Pnc<l0a.m01i dree s(p5e mctgiv/kegly).) Irne dourcael dg lfuacstoisneg tbolloeoradn gcleu cteosste, 5le0v0e ml ign/k ngo drmosael bsilgonoidfi cgalunctloys ere ldeuvceel da tb 2lo ho da ngdlu 3c ohs e(P le<v0e.0l 5( Pa<n0d. 0P5)< a0t. 021 hr ebsupte 1c 0ti0v0e mlyg) /wkhge dreoases sgilgibneifniccalanmtlyid ree d(5u mcegd/ kisg )a lssiog nciofincsaindtelrye dre edxutcreemd egllyu csiogsnei fliecvaenlt awt itehv eFr yv ahlouuer= a23ft.e83r aadnmd iPn ivstarlauteio=n0.. 0O00v1e rianl lo rtiaml eg luefcfoescet tolerance test. Conclusion: These findings suggest that the plant may be a potential source for the development of new oral hypoglycemic agent.
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    ABSTRACT: The polar constituents of peels from Citrus limetta variety Risso (Rutaceae) were investigated by a combination of two complementary chromatographic techniques consisting of preparative high-speed countercurrent chromatography (HSCCC), and off-line LC–ESI–MS/MS analysis to design a two-dimensional metabolite profile. Countercurrent chromatography (CCC) using solely immiscible solvent systems allowed the fractionation of principal components and an enrichment of minor concentrated metabolites from a crude polar solvent partition of C. limetta peels for subsequent structural identification by LC–ESI–MS/MS analysis. The combination of two very different chromatographic techniques resulted in lower detection limits for electrospray mass-spectrometry and revealed eighty-five compounds, including three abscisic acid derivatives, five limonoid glycosides, twenty-six dihydro-cinnamic and cinnamic acid glycosides, eleven flavanone glycosides, seven flavone glycosides, seventeen flavonol glycosides, including limocitrol and limocitrin derivatives. As a chemocharacteristic for C. limetta metabolites, many of the detected structures were linked to single and multiple 3-hydroxy-3-methyl-glutaryl (HMG) substitutions. C. limetta peels are a by-product of juice production, and not only the antioxidant fractions but also some of the fortified compounds could be used for food and pharmaceutical purposes.
    Food Chemistry 09/2014; 158:139–152. DOI:10.1016/j.foodchem.2014.02.077 · 3.39 Impact Factor