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Anti-Cancer Effect of Datura innoxia P.mill. Leaf Extract in vitro through Induction of Apoptosis in Human Colon Adenocarcinoma and Larynx Cancer Cell Lines.

J. Pharmacy Res 01/2010; 3:1485-1488.

ABSTRACT Native medicinal plants are traditionally used to prevent and treat a variety of diseases, including cancer. These herbal preparations alleged to have many
biological activities, such as stimulation or suppression of immune responses and antiprolifeative effects on cancer cells. In the present study, we
investigated the effects of methanolic leaf extracts from Datura innoxia, on the growth of human colon adenocarcinoma (HCT 15) and human larynx cancer
cell lines (Hep-2). The methanolic leaf extracts from Datura innoxia potently inhibited growth of both cell lines in a dose dependent manner with IC50 value
300μg/ml and for HCT 15 and Hep-2 cells respectively. The results showed the anti-cancer action is due to nuclear fragmentation and condensation,
associated with the appearance of apoptosis. In addition, Datura innoxia treated HCT 15 and Hep-2 cells had decrease expression of antiapoptotic Bcl-2
protein. These results demonstrated that Datura innoxia inhibits the proliferation of HCT 15 and Hep-2 cells through induction of apoptosis.

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