Alteration in nitrogen metabolism and plant growth during different developmental stages of green gram ( Vigna radiata L.) in response to chlorpyrifos

Acta Physiologiae Plantarum (Impact Factor: 1.31). 01/2011; Acta Physiologiae Plantarum (26 April 2011), pp. 1-8. doi:10.1007/s11738-011-0772-2 Key: citeulike:9246107. DOI: 10.1007/s11738-011-0772-2

ABSTRACT Chlorpyrifos is a widely used broad-spectrum organophosphate insecticide in the agricultural practice. However, extensive use of this insecticide may lead to its accumulation in ecosystem, thus inducing the toxicity to crops and vegetables. To assess chlorpyrifos-induced toxicity in plants, we performed the experiment focusing on the growth and nitrogen metabolism of green gram plant (Vigna radiata L.). 20-days-old plants were subjected to chlorpyrifos at concentrations ranging from 0 to 1.5 mM through foliar spray in the field condition. Variation in root and shoot length, activities of nitrate reductase (NR) and content of nitrate, sugar, soluble amino acid and soluble protein were studied at preflowering (5 day after treatment, DAT), flowering (10 DAT) and postflowering (20 DAT) stages of plant development. Of the various concentrations of chlorpyrifos, 0.6 and 1.5 mM showed comparatively more severe toxicity to green gram plants by decreasing root and shoot length, nitrate, NR, soluble sugar and protein content where as at low concentration (0.3 mM) of chlorpyrifos proved stimulant for same parameter. Increase in soluble amino acid was observed in age and dose dependent manner. These results reflect strong parallelism between growth and biochemical activities of the model plant. Further lower dose of chlorpyrifos proved as stimulant where as at higher concentration proved detrimental for growth and nitrogen metabolism.

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    ABSTRACT: This study aims to evaluate the effect of chlorpyrifos on several metabolic and stress related parameters of Vigna radiata L. Twenty-day-old plants were exposed to several concentrations of chlorpyrifos, ranging from 0 to 1.5 mM through foliar spray in the field condition. Analyses were done at pre-flowering (Day 5), flowering (Day 10), and post-flowering (Day 20) stages after the treatment. Lipid peroxidation rate, proline, dehydroascorbate, oxidized and total glutathione were all ascended. Chlorpyrifos enhanced lipid peroxidation rate and proline content with 1.5 mM at Day 20 whereas dehydroascorbate, oxidized and total glutathione were increased in 1.5 mM at Day 10. However, dose dependence significantly declined in content of ascorbate and reduced glutathione levels were observed at all growth stages. Among the enzymatic antioxidants, activities of superoxide dismutase, ascorbate peroxidase and glutathione reductase enhanced significantly in all the concentrations at Day 10. Maximum catalase activity was observed at Day 10 in control and it declined thereafter. The above results clearly depicted the provoked state of oxidative stress under chlorpyrifos exposure in Vigna radiata L. and therefore can be used to evaluate the degree of insecticide contamination to plant, which may be serving as biomarker in Vigna radiata L.
    International journal of Environmental Science and Technology 06/2012; · 1.84 Impact Factor


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Jun 3, 2014