Stamenkovic, I., Sgroi, D., Aruffo, A., Sy, M.S. & Anderson, T. The B lymphocyte adhesion molecule CD22 interacts with leukocyte common antigen CD45RO on T cells and 2,6 sialyltransferase, CD75, on B cells. Cell 66, 1133−1144

Department of Pathology, Massachusetts General Hospital, Charlestown.
Cell (Impact Factor: 32.24). 10/1991; 66(6):1133-44. DOI: 10.1016/0092-8674(91)90036-X
Source: PubMed


Functional maturation of B lymphocytes correlates with expression of the B lineage-specific cell surface glycoprotein CD22. Two CD22 polypeptides have been characterized and suggested to play a role in B cell-B cell interaction as well as in B cell adhesion to monocytes. In this work we provide evidence that CD22 is directly involved in the cognate interaction between B and T cells. One of the two CD22 polypeptides, CD22 beta, interacts with a specific ligand on a subpopulation of CD4+ T cells. Our results suggest that the T cell ligand of CD22 is CD45RO, an isoform of the leukocyte common antigen class of phosphotyrosine phosphatases associated with the helper T cell phenotype. We further demonstrate that CD22 recognizes a second ligand, CD75, expressed predominantly on activated B cells and shown to be a cell surface alpha 2-6 sialyltransferase.

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    • "CD22 is closely related to leukocyte and B lymphocyte and the immune cells are assumed to be strongly associated with the ovulation processes. CD22 was expressed in B-lineage acute leukemia (Toba et al., 2003) and as a B lymphocyte adhesion molecule, CD22 was found to interact with leukocyte common antigen CD45RO on T cells (Stamenkovic et al., 1991). In rabbits, T lymphocytes dramatically increased in the uterus after ovulation, and both before and after ovulation, the cells were observed frequently in mucosa of the oviduct, cervix, and vagina (Gu et al., 2005). "
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    • ". LASALLE J.M. & HAFLER D.A. (1991) "
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    ABSTRACT: Differences in levels of specific enzymes utilized in intracellular signalling could be a factor in the distinct signalling properties observed in memory and naive T cells. We have studied the expression of both classical and non-classical protein kinase of C (PKC) isoenzymes in CD45RA and CD45RO cells using a combination of Western blot and flow cytometric analysis. These data indicate that CD45RA cells express higher levels of PKC alpha, PKC beta and PKC delta than CD45RO cells. In addition, CD45RA+ cells show greater proliferative activity when stimulated with phorbol myristate acetate (PMA) and calcium ionophore than their CD45RO+ counterparts. Variations in the levels of these isoenzymes could be implicated in functional differences, such as proliferation and cytokine production, in these cell subsets.
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    • "On T cells, various isoforms distinguish T helper cell subsets (Bottomly et al., 1989) and resting or activated T cells (Byrne et al., 1988). Physiological ligands of CD45 have not been definitively established, but CD45 interacts with the B cell antigen CD22 (Stamenkovic et al., 1991), which binds to many plasma membrane proteins that are sialylated (Sgroi et al., 1993). "
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