Mitochondrial abnormalities in the postviral fatigue syndrome.
ABSTRACT We have examined the muscle biopsies of 50 patients who had postviral fatigue syndrome (PFS) for from 1 to 17 years. We found mild to severe atrophy of type II fibres in 39 biopsies, with a mild to moderate excess of lipid. On ultrastructural examination, 35 of these specimens showed branching and fusion of mitochondrial cristae. Mitochondrial degeneration was obvious in 40 of the biopsies with swelling, vacuolation, myelin figures and secondary lysosomes. These abnormalities were in obvious contrast to control biopsies, where even mild changes were rarely detected. The findings described here provide the first evidence that PFS may be due to a mitochondrial disorder precipitated by a virus infection.
SourceAvailable from: Sharni Lee Hardcastle[Show abstract] [Hide abstract]
ABSTRACT: Background Several diagnostic definitions are available for Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME) that varies significantly in their symptom criteria. This pilot study was conducted to determine whether simple biological and clinical measures differed between CFS/ME patients meeting the 1994 Centres for Disease Control and Prevention (CDC) criteria, the International Consensus Criteria (ICC), as well as healthy controls. Methods A total of 45 CFS/ME patients and 30 healthy controls from the South East Queensland region of Australia provided a blood sample, reported on their current symptoms, as well as aspects of their physical and social health using the Short-Form Health Survey (SF-36), and the World Health Organisation Disability Adjustment Schedule 2.0 (WHO DAS 2.0). Differences were examined using independent sample t-testing. Results Patients fulfilling the ICC definition reported significantly lower scores (p < 0.05) for physical functioning, physical role, bodily pain, and social functioning than those that only fulfilled the 1994 CDC definition. ICC patients reported significantly greater (p < 0.05) disability across all domains of the WHO DAS 2.0. Conclusions These preliminary findings suggest that the ICC identifies a distinct subgroup found within patients complying with the 1994 CDC definition, with more severe impairment to their physical and social functioning.Health and Quality of Life Outcomes 04/2014; 12(1):64. DOI:10.1186/1477-7525-12-64 · 2.10 Impact Factor
Conference Paper: Electrical characterization of a C-Element with LiChEn[Show abstract] [Hide abstract]
ABSTRACT: This demonstration presents the use of the Library Characterization Environment (LiChEn) for characterizing asynchronous standard cells. The tool was employed for the electrical characterization of a library with over five hundred asynchronous standard cells. In this work, a case study of a fundamental asynchronous component, the C-Element, will be presented to validate the use of the tool. LiChEn was designed due to the necessity of automating the process of characterizing asynchronous standard cells. Albeit this task can be done with tools from industrial EDA vendors, the use of these proved to require laborious manual work. These tools were designed for characterizing standard cells for synchronous systems and usually fail to recognize complex asynchronous logic. Moreover, asynchronous components are not available off the shelf in typical standard cell libraries, which constrains the asynchronous paradigm for full-custom approaches. As asynchronous techniques gain relevance in the research community, LiChEn can present a practical solution for a wider adoption of such techniques, by allowing an automated characterization of asynchronous standard cells.Electronics, Circuits and Systems (ICECS), 2012 19th IEEE International Conference on; 01/2012
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ABSTRACT: Although Myalgic Encephalomyelitis (ME) and Chronic Fatigue Syndrome (CFS) are used interchangeably, the diagnostic criteria define two distinct clinical entities. Cognitive impairment, (muscle) weakness, circulatory disturbances, marked variability of symptoms, and, above all, post-exertional malaise: a long-lasting increase of symptoms after a minor exertion, are distinctive symptoms of ME. This latter phenomenon separates ME, a neuro-immune illness, from chronic fatigue (syndrome), other disorders and deconditioning. The introduction of the label, but more importantly the diagnostic criteria for CFS have generated much confusion, mostly because chronic fatigue is a subjective and ambiguous notion. CFS was redefined in 1994 into unexplained (persistent or relapsing) chronic fatigue, accompanied by at least four out of eight symptoms, e.g., headaches and unrefreshing sleep. Most of the research into ME and/or CFS in the last decades was based upon the multivalent CFS criteria, which define a heterogeneous patient group. Due to the fact that fatigue and other symptoms are non-discriminative, subjective experiences, research has been hampered. Various authors have questioned the physiological nature of the symptoms and qualified ME/CFS as somatization. However, various typical symptoms can be assessed objectively using standardized methods. Despite subjective and unclear criteria and measures, research has observed specific abnormalities in ME/CFS repetitively, e.g., immunological abnormalities, oxidative and nitrosative stress, neurological anomalies, circulatory deficits and mitochondrial dysfunction. However, to improve future research standards and patient care, it is crucial that patients with post-exertional malaise (ME) and patients without this odd phenomenon are acknowledged as separate clinical entities that the diagnosis of ME and CFS in research and clinical practice is based upon accurate criteria and an objective assessment of characteristic symptoms, as much as possible that well-defined clinical and biological subgroups of ME and CFS patients are investigated in more detail, and that patients are monitored before, during and after interventions with objective measures and biomarkers.Frontiers in Physiology 03/2014; 5:109. DOI:10.3389/fphys.2014.00109