Article

Nucleotide sequence of the gene encoding infectious laryngotracheitis virus glycoprotein B.

CSIRO Division of Animal Health, Animal Research Laboratory, Parkville, VIC, Australia.
Virology (impact factor: 3.35). 10/1991; 184(1):404-10. pp.404-10
Source: PubMed

ABSTRACT The nucleotide sequence of the infectious laryngotracheitis virus (ILTV) gene encoding the 205K complex glycoprotein (gp205) was determined. The gene is contained within a 3-kb EcoRI restriction fragment mapping at approximately map coordinates 0.23 to 0.25 in the UL region of the ILTV genome and is transcribed from right to left. Nucleotide sequence analysis of the DNA fragment identified a single, long open reading frame capable of encoding 873 amino acids. The predicted precursor polypeptide derived from this open reading frame would have a calculated Mr of 98,895 Da and contains nine potential glycosylation sites. Hydropathic analysis indicates the presence of an amino terminal hydrophobic sequence and hydrophobic carboxyl terminal domain which may function as a signal peptide and a membrane anchor sequence, respectively. Comparison of the predicted ILTV gp205 protein sequence with those of other herpesviruses revealed a significant sequence similarity with gB-like glycoproteins. Extensive homology was observed throughout the molecule except for the amino and carboxyl termini. The high homology in predicted primary and secondary structures is consistent with the essential role of the gB family of proteins for viral infectivity and pathogenesis.

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Keywords

3-kb EcoRI restriction fragment
 
amino terminal hydrophobic sequence
 
carboxyl termini
 
DNA fragment
 
encoding 873 amino acids
 
essential role
 
gB family
 
gB-like glycoproteins
 
herpesviruses
 
hydrophobic carboxyl terminal domain
 
infectious laryngotracheitis virus
 
membrane anchor sequence
 
nucleotide sequence
 
Nucleotide sequence analysis
 
open reading frame
 
open reading frame capable
 
potential glycosylation sites
 
predicted ILTV gp205 protein sequence
 
secondary structures
 
significant sequence similarity
 

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